Methylated APC and RASSF1A in multiple specimens contribute to the differential diagnosis of patients with undetermined solitary pulmonary nodules
Methylated APC and RASSF1A in multiple specimens contribute to the differential diagnosis of patients with undetermined solitary pulmonary nodules
复制标题
多个样本中的甲基化 APC 和 RASSF1A 有助于鉴别诊断未确定的孤立性肺结节患者
DOI：
10.3978/j.issn.2072-1439.2015.01.24
复制
发表时间：
2015-03-01
影响因子：
2.5
通讯作者：
Pan, Shiyang
中科院分区：
文献类型：
--
作者：
Gao, Li;Xie, Erfu;Pan, Shiyang
背景：肿瘤抑制基因（TSG）启动子甲基化失活在包括肺癌在内的多种肿瘤中均有报道。本研究旨在检测早期肺癌患者肿瘤组织、血清和血浆中APC和RASSF 1A基因的甲基化水平。从活检组织、血清和血浆中提取DNA样品，并在亚硫酸氢盐转化后进行APC和RASSF 1A的QMSP。根据病理报告，89例患者包括58例I期肺癌和31例良性肺部疾病。结果：APC和RASSF 1A基因甲基化率在肿瘤活检组织中分别为59.0%和66.1%，在血清中分别为42.5%和52.5%，在血浆中分别为24.1%和43.1%。RASSF 1A在不同组织中的表达在恶性肿瘤组与良性肿瘤组之间差异均有显著性（P
Background: Inactivation of tumor-suppressor gene (TSG) by promoter hypermethylation has been reported in many tumor types, including lung cancer. This study was designed to determine the methylated APC and RASSF1A genes in tumor tissue, serum and plasma of patients with early stage lung cancer.Methods: Eighty-nine patients with undetermined solitary pulmonary nodules detected upon CT-scan were recruited in this study. DNA samples were extracted from biopsy tissues, serum and plasma and QMSP of APC and RASSF1A was carried out after bisulfite conversion. The 89 patients consist of 58 stage I lung cancer patients and 31 benign lung disease according to pathological report. Twenty-six cancer patients had matched biopsy tumor tissue, serum and plasma samples.Results: The methylation rates of APC and RASSF1A were 59.0% and 66.1% in biopsy tissues, 42.5% and 52.5% in serum, and 24.1% and 43.1% in plasma of cancer patients. For RASSF1A, different samples all showed a significant difference between cancer group and benign group (P