Enrichment of native plastic-associated biofilm communities to enhance polyester degrading activity
Enrichment of native plastic-associated biofilm communities to enhance polyester degrading activity
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DOI：
10.1111/1462-2920.16466
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发表时间：
2023-07-28
影响因子：
5.1
通讯作者：
McCarthy, Ronan R. R.
中科院分区：
文献类型：
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作者：
Howard, Sophie A. A.;Carr, Clodagh M. M.;McCarthy, Ronan R. R.
塑料污染是一个日益严重的世界性问题，迫切需要解决。虽然全球的回收率正在提高，但只有9%的塑料垃圾被回收，由于回收塑料的成本和下游使用有限，需要一种替代方案。在这里，我们发现发泡聚苯乙烯(EPS)促进了高水平的细菌生物膜的形成，并寻找环境EPS废物来表征这些本土群落。我们证明了EPS附着的群落具有有限的塑料降解活性。然后，我们进行了一项长期的富集化实验，通过限制碳的可获得性，使废塑料成为唯一的碳源，对这些群落施加了强大的选择压力。与开始的细菌群落相比，产生的丰富细菌群落中有7个提高了塑料降解活性。在七个浓缩群落中的六个群落中，斯图泽里假单胞菌被确定为最强的聚酯降解物。对斯图泽里肺炎杆菌的一个分离株进行了测序，发现了两种可能的聚酯酶和一种可能的MHETase。这表明，与废塑料相关的生物膜是具有塑料降解潜力的细菌的来源，通过选择压力和进一步的体外浓缩实验，可以释放这种潜力，从而产生比自然更好的生物降解群落。
Plastic pollution is an increasing worldwide problem urgently requiring a solution. While recycling rates are increasing globally, only 9% of all plastic waste has been recycled, and with the cost and limited downstream uses of recycled plastic, an alternative is needed. Here, we found that expanded polystyrene (EPS) promoted high levels of bacterial biofilm formation and sought out environmental EPS waste to characterize these native communities. We demonstrated that the EPS attached communities had limited plastic degrading activity. We then performed a long-term enrichment experiment where we placed a robust selection pressure on these communities by limiting carbon availability such that the waste plastic was the only carbon source. Seven of the resulting enriched bacterial communities had increased plastic degrading activity compared to the starting bacterial communities. Pseudomonas stutzeri was predominantly identified in six of the seven enriched communities as the strongest polyester degrader. Sequencing of one isolate of P. stutzeri revealed two putative polyesterases and one putative MHETase. This indicates that waste plastic-associated biofilms are a source for bacteria that have plastic-degrading potential, and that this potential can be unlocked through selective pressure and further in vitro enrichment experiments, resulting in biodegradative communities that are better than nature.