Prognostic value of vascular endothelial growth factors A and C in oral squamous cell carcinoma.
Prognostic value of vascular endothelial growth factors A and C in oral squamous cell carcinoma.
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DOI：
10.1111/jop.12167
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发表时间：
2014-08
期刊：
影响因子：
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通讯作者：
M. Yanase;K. Kato;K. Yoshizawa;Natsuyo Noguchi;H. Kitahara;Hiroyuki Nakamura
中科院分区：
文献类型：
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作者：
M. Yanase;K. Kato;K. Yoshizawa;Natsuyo Noguchi;H. Kitahara;Hiroyuki Nakamura
背景血管内皮生长因子（VEGF）家族成员在血管生成和血管化中起主要作用。VEGF-A通过刺激肿瘤血管内皮细胞的生长来促进肿瘤血管生成。此外，VEGF-C已被鉴定为肿瘤和淋巴结转移中淋巴管生成的有效诱导剂。以往的研究探讨了口腔鳞状细胞癌（OSCC）中VEGF-A和VEGF-C的表达与临床病理因素之间的关系，但结果相互矛盾。在这项研究中，我们研究了VEGF-A和VEGF-C表达与OSCC临床病理因素和预后的关系。方法采用免疫组化和酶联免疫吸附试验（ELISA）检测61例口腔鳞癌组织和3株人口腔癌细胞系（OSC-19、OSC-20和HOC 313）中VEGF-A和VEGF-C的表达。我们还确定了5年生存率与临床病理因素之间的关系，如TNM分类（国际癌症控制联盟，UICC），淋巴结转移，复发，组织分化，位置和浸润方式。结果VEGF-A表达与淋巴结转移密切相关。VEGF-C表达与淋巴结转移、复发和5年生存率相关。多因素分析显示VEGF-C是影响口腔鳞癌患者预后的独立因素。与OSC-19和OSC-20细胞相比，HOC 313细胞中VEGF-C表达显著上调。结论VEGF-C可能是口腔鳞癌预后、淋巴结转移和复发的预测因子。此外，VEGF-C可能是OSCC患者新疗法开发的重要因素。
BACKGROUND Vascular endothelial growth factor (VEGF) family members play a major role in angiogenesis and vascularization. VEGF-A promotes tumor angiogenesis by stimulating the growth of tumor vascular endothelial cells. In addition, VEGF-C has been identified as a potent inducer of lymphangiogenesis in tumor and lymph node metastasis. Previous studies have investigated the association between clinicopathological factors and the expression of VEGF-A and VEGF-C in oral squamous cell carcinoma cancer (OSCC), but the results are contradictory. In this study, we investigated the relationship between VEGF-A and VEGF-C expression and OSCC clinicopathological factors and prognosis. METHODS Expression of VEGF-A and VEGF-C was evaluated in surgical specimens from 61 patients with OSCC and three human oral cancer cell lines (OSC-19, OSC-20 and HOC313) by immunohistochemical staining and enzyme-linked immunosorbent assay, respectively. We also determined the relationship between the 5-year survival rate and clinicopathological factors, such as TNM classification (Union for International Cancer Control, UICC), lymph node metastasis, recurrence, histological differentiation, location, and mode of invasion. RESULTS VEGF-A expression correlated significantly with lymph node metastasis. VEGF-C expression was associated with lymph node metastasis, recurrence, and a poorer 5-year survival rate. A multivariate analysis demonstrated that VEGF-C is an independent prognostic factor for patients with OSCC. VEGF-C expression was significantly up-regulated in HOC313 cells compared to OSC-19 and OSC-20 cells. CONCLUSIONS These results indicate that VEGF-C may be a predictive factor for OSCC outcome, lymph node metastasis, and recurrence. Moreover, VEGF-C may be an important factor in the development of new therapies for OSCC patients.