Characterization of swine testicular cell line as immature porcine Sertoli cell line
Characterization of swine testicular cell line as immature porcine Sertoli cell line
复制标题
猪睾丸细胞系作为未成熟猪支持细胞系的表征
DOI：
10.1007/s11626-015-9994-8
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发表时间：
2016-04-01
影响因子：
2.1
通讯作者：
Li, Fenge
中科院分区：
文献类型：
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作者：
Ma, Changping;Song, Huibin;Li, Fenge
猪睾丸（ST）细胞系是从猪胎儿睾丸中分离得到的细胞系，已广泛应用于猪病毒感染相关的生物医学研究领域。然而，ST细胞在公猪繁殖研究中的潜在益处和利用尚未得到充分探索。由于猪胎儿睾丸主要含有间质细胞、支持细胞、生殖细胞和管周肌样细胞等多种类型的细胞，因此有必要明确ST细胞系的细胞类型。本研究通过对支持细胞的卫星核体形态学特征的分析，以及对支持细胞两个标志物（雄激素结合蛋白（ABP）和Fas配体（FASL））蛋白表达的检测，初步鉴定ST细胞系为支持细胞的集合体。通过检测抗苗勒管激素（anti-Mullerian hormone，AMH）、角蛋白18（keratin 18，KRT 18）和促卵泡激素受体（follicle-stimulating hormone receptor，FSHR）的表达，进一步证实ST细胞为未成熟的支持细胞。ST细胞是一批未成熟的支持细胞，可作为研究支持细胞功能和成熟的良好实验材料。
Swine testicular (ST) cell line is isolated from swine fetal testes and has been widely used in biomedical research fields related to pig virus infection. However, the potential benefit and utilization of ST cells in boar reproductive studies has not been fully explored. As swine fetal testes mainly contain multiple types of cells such as Leydig cells, Sertoli cells, gonocytes, and peritubular myoid cells, it is necessary to clarify the cell type of ST cell line. In this study, we identified ST cell line was a collection of Sertoli cells by analyzing the unique morphological characteristic with satellite karyosomes and determining the protein expression of two markers (androgen-binding protein, ABP; Fas ligand, FASL) of Sertoli cells. Then ST cells were further confirmed to be immature Sertoli cells by examining the expression of three markers (anti-Mullerian hormone, AMH; keratin 18, KRT18; follicle-stimulating hormone receptor, FSHR). In conclusion, ST cells are a collection of immature Sertoli cells which can be good experimental materials for the researches involved in Sertoli cell functions and maturation, or even in boar reproductions.