Increased activity of only an individual non-regulated enzyme fructose-1,6-bisphosphate aldolase in Anabaena sp strain PCC 7120 stimulates photosynthetic yield
Increased activity of only an individual non-regulated enzyme fructose-1,6-bisphosphate aldolase in Anabaena sp strain PCC 7120 stimulates photosynthetic yield
复制标题
鱼腥藻属中仅单个非调节酶果糖-1,6-二磷酸醛缩酶的活性增加。
DOI：
10.1007/s11738-008-0182-2
复制
发表时间：
2008-11-01
影响因子：
2.6
通讯作者：
Wang, Quanxi
中科院分区：
文献类型：
--
作者：
Ma, Weimin;Wei, Lanzhen;Wang, Quanxi
The goal of this study was to investigate the contribution of increased activity of individual non-regulated enzymes in the Calvin cycle to improve photosynthetic yield. Two non-regulated enzymes, rice fructose-1,6-bisphosphate aldolase (FBA) and spinach triosephosphate isomerase (TPI), were individually cloned and overexpressed in the cyanobacterium Anabaena sp. strain PCC 7120 cells. The enzyme activity and the photosynthetic yield, as reflected by the cell growth rate, photosynthetic oxygen evolution and dry cellular weight, were measured and compared between the wild-type and transgenic cells harboring either FBA or TPI. Though the activity of these two individual non-regulated enzymes was similarly increased in the corresponding transgenic cells, the contributions of each enzyme on the amount of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), reflected by the levels of Rubisco large subunit, and the photosynthetic yield were different. Transgenic cells, carrying FBA, showed an evident increase in Rubisco amount and photosynthetic yield, while there was no increase in cells harboring TPI. This indicates that the contributions of non-regulated enzymes in the Calvin cycle on photosynthetic yield differed and firstly reveals that increased activity of only a single non-regulated enzyme in transgenic cells markedly improves the photosynthetic yield via stimulating the amount of Rubisco and consequently accelerating the ribulose-1,5-bisphosphate (RuBP) regeneration rate.
