The host-pathogen interaction between wheat and yellow rust induces temporally coordinated waves of gene expression.
The host-pathogen interaction between wheat and yellow rust induces temporally coordinated waves of gene expression.
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DOI：
10.1186/s12864-016-2684-4
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发表时间：
2016-05-20
期刊：
影响因子：
4.4
通讯作者：
Saunders DG
中科院分区：
文献类型：
--
作者：
Dobon A;Bunting DC;Cabrera-Quio LE;Uauy C;Saunders DG
了解植物和病原体在宿主-病原体相互作用过程中如何调节基因表达是揭示调控疾病进展的分子机制的关键。测序技术的最新进展为解码这种相互作用的复杂性提供了新的机会。在这项研究中，我们使用基于RNA的测序方法（RNA-seq）来评估小麦条锈菌病原体条锈菌f.三化螟（PST）与寄主的关系。我们对感染PST的易感和抗性小麦宿主进行了详细的RNA-seq时间过程。本研究（i）定义了PST及其小麦宿主的全局基因表达谱，（ii）大大改进了PST的基因模型，（iii）评估了几种用于量化PST和小麦全局基因表达的程序的实用性，以及（iv）确定了宿主和病原体中差异表达基因的簇。通过关注易感和抗性宿主中防御反应的组成部分，我们能够可视化PST感染对各种防御成分和宿主免疫受体表达的影响。我们的数据显示，顺序，时间协调激活和抑制一套免疫反应调节剂的表达，兼容和不兼容的相互作用之间的变化。这些发现为更好地理解PST如何引起疾病提供了框架，并支持PST可以抑制小麦中防御成分的表达以成功地定殖易感宿主的想法。本文的在线版本（doi：10.1186/s12864-016-2684-4）包含补充材料，可供授权用户使用。
Understanding how plants and pathogens modulate gene expression during the host-pathogen interaction is key to uncovering the molecular mechanisms that regulate disease progression. Recent advances in sequencing technologies have provided new opportunities to decode the complexity of such interactions. In this study, we used an RNA-based sequencing approach (RNA-seq) to assess the global expression profiles of the wheat yellow rust pathogen Puccinia striiformis f. sp. tritici (PST) and its host during infection. We performed a detailed RNA-seq time-course for a susceptible and a resistant wheat host infected with PST. This study (i) defined the global gene expression profiles for PST and its wheat host, (ii) substantially improved the gene models for PST, (iii) evaluated the utility of several programmes for quantification of global gene expression for PST and wheat, and (iv) identified clusters of differentially expressed genes in the host and pathogen. By focusing on components of the defence response in susceptible and resistant hosts, we were able to visualise the effect of PST infection on the expression of various defence components and host immune receptors. Our data showed sequential, temporally coordinated activation and suppression of expression of a suite of immune-response regulators that varied between compatible and incompatible interactions. These findings provide the framework for a better understanding of how PST causes disease and support the idea that PST can suppress the expression of defence components in wheat to successfully colonize a susceptible host. The online version of this article (doi:10.1186/s12864-016-2684-4) contains supplementary material, which is available to authorized users.