POSTNATAL-DEVELOPMENT OF THE EXCITATORY AMINO-ACID SYSTEM IN VISUAL-CORTEX OF THE RAT - CHANGES IN LIGAND-BINDING TO NMDA, QUISQUALATE AND KAINATE RECEPTORS
POSTNATAL-DEVELOPMENT OF THE EXCITATORY AMINO-ACID SYSTEM IN VISUAL-CORTEX OF THE RAT - CHANGES IN LIGAND-BINDING TO NMDA, QUISQUALATE AND KAINATE RECEPTORS
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DOI：
10.1016/0736-5748(90)90011-p
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发表时间：
1990-01-01
影响因子：
1.8
通讯作者：
WOLFF, JR
中科院分区：
文献类型：
--
作者：
ERDO, SL;WOLFF, JR
在2-360日龄大鼠视觉皮质全匀浆中，观察了n -甲基- d -天冬氨酸（NMDA）、准质酸和海碱酸受体位点的配体在出生后的发育。选用[3H]CPP(3-（2-羧基哌嗪-4-基）-丙基-1-膦酸、[3H]AMPA （rs - α -氨基-3-羟基-5-甲基异恶唑-4-丙酸）和[3H]KA （kainic酸）作为选择性配体。新生儿CPP的结合较低，从出生后第二周开始迅速增加，在第2周至第3周达到最大值，然后缓慢下降，直到1岁。与此相反，AMPA和kainate的结合在围产期较高，在出生后第6天迅速增加，达到早期最大值，然后逐渐下降到3-4周龄时达到成体值。这些与年龄相关的变化源于结合位点密度的改变，在AMPA的情况下，这伴随着结合亲和力的增加。结果表明，在未成熟的大脑皮层中，NMDA受体可能主要参与突触传递，而准质酸受体和盐酸盐受体可能发挥其他作用（如营养作用）。
The postnatal development of the ligand binding to N-methyl-D-aspartate (NMDA), quisqualate and kainate receptor sites was examined in whole homogenates of the visual cortex of rats, aged 2-360 days. As selective ligands, [3H]CPP (3-(2-carboxypyperazine-4-yl)-propyl-1-phosphonic acid, [3H]AMPA (RS-alpha-amino-3-hydroxy-5-methyl-isoxazole-4-propionic acid)) and [3H]KA (kainic acid) were used, respectively. The binding of CPP was low in newborns, rapidly increased from the second postnatal week, reached its maximum between weeks 2 and 3, then slowly declined up to the age of 1 year. In contrast, the binding of AMPA and kainate was high perinatally, increased rapidly up to day 6 after birth to reach an early maximum value, then gradually decreased to adult values which were attained at an age of 3-4 weeks. These age-related changes were derived from alterations in the density of binding sites, which, in the case of AMPA, was accompanied by an increase in binding affinity. The results, compared with the developmental time-course of excitatory synapses, indicate that, in the immature cerebral cortex, NMDA receptors may be primarily involved in synaptic transmission, whereas quisqualate and kainate receptors may play some other (e.g. trophic) roles.