RAPID ISOLATION OF PANCREATIC-ISLETS FROM COLLAGENASE DIGESTED PANCREAS BY SEDIMENTATION THROUGH PERCOLL AT UNIT GRAVITY
RAPID ISOLATION OF PANCREATIC-ISLETS FROM COLLAGENASE DIGESTED PANCREAS BY SEDIMENTATION THROUGH PERCOLL AT UNIT GRAVITY
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DOI：
10.1016/0006-291x(77)91185-8
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发表时间：
1977-01-01
影响因子：
3.1
通讯作者：
PERTOFT, H
中科院分区：
文献类型：
--
作者：
BUITRAGO, A;GYLFE, E;PERTOFT, H
使用密度为1.045 μ g/ml的Percoll溶液，通过单位重力沉降从胶原酶消化的人和ob/ob小鼠胰腺中分离胰岛和外分泌组织。发现来自小鼠的大多数外分泌组织的密度范围为1.015-1.045 g/ml，而更致密的胰岛位于1.065-1.070 g/ml的较窄范围内。从每个小鼠胰腺获得多达400个胰岛，从4g人胰腺获得140个胰岛;分离的胰岛基本上不受外分泌组织的污染。葡萄糖刺激的胰岛素释放是相同的，无论小鼠胰岛分离与Percoll或没有。该方法的简单性使其适合于大规模胰岛分离，这是通过胰岛移植治疗糖尿病的潜在重要性的特征。
A Percoll solution with a density of 1.045 g/ml was used to separate pancreatic islets and exocrine tissue from collagenase-digested human and ob/ob mouse panceases by sedimentation at unit gravity. Most exocrine tissue from the mouse was found to range in density from 1.015-1.045 g/ml, whereas the denser islets lay in a narrower range of 1.065-1.070 g/ml. Up to 400 islets were obtained from each mouse pancreas and 140 islets from 4 g of human pancreas; the isolated islets were essentially free from contamination with exocrine tissue. Glucose-stimulated insulin release was the same whether the mouse islets were isolated with or without Percoll. The simplicity of the method makes it suitable for large-scale islet isolation, a feature of potential importance for the treatment of diabetes by islet transplantation.