Molecular Characterization of Vancomycin-Resistant Enterococci in a Chinese Hospital Between 2003 and 2009
Molecular Characterization of Vancomycin-Resistant Enterococci in a Chinese Hospital Between 2003 and 2009
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DOI：
10.1089/mdr.2011.0025
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发表时间：
2011-09-01
影响因子：
2.6
通讯作者：
Wang, Hui
中科院分区：
文献类型：
--
作者：
Liu, Yingmei;Cao, Bin;Wang, Hui
从2003年6月至2009年12月，从中国北京一家拥有1,500张床位的三级护理教学医院住院患者的临床标本中培养出98株万古霉素耐药肠球菌（VRE）。分离株的特点是脉冲场凝胶电泳和多位点序列分型。我们调查了vanA基因簇的结构和分布的毒力标记esp，hyl，gelE，asa 1，和cylA的聚合酶链反应。我们的研究结果表明，多位点序列分型揭示了五个新的序列类型和一个新的等位基因。VRE屎（VREfm）分离株在其vanA簇类型和毒力基因的存在下是异质性的。我们还观察到VREfm分离株的基因型和表型之间的不一致。在我们医院爆发的VREfm似乎是多克隆的，而VRE粪表征表明一个特定的克隆传播。2007年后，VRE faecalis被VREfm完全取代，VREfm此后一直是我院的优势菌种。VRE在我们医院似乎处于一种进化的潮流中。
From June 2003 to December 2009, 98 isolates of vancomycin-resistant enterococci (VRE) were cultured from clinical specimens taken from patients admitted to a 1,500-bed tertiary-care teaching hospital in Beijing, China. Isolates were characterized by pulsed-field gel electrophoresis and multilocus sequence typing. We investigated the structure of the vanA gene cluster and the distribution of the virulence markers esp, hyl, gelE, asa1, and cylA by polymerase chain reaction. Our results indicate that multilocus sequence typing revealed five novel sequence types and one new allele. VRE faecium (VREfm) isolates were heterogeneous in their vanA cluster types and in the presence of virulence genes. We also observed inconsistency between genotype and phenotype in VREfm isolates. The outbreak with VREfm in our hospital appears polyclonal, whereas VRE faecalis characterization indicated dissemination of a particular clone. After 2007, VRE faecalis was completely replaced by VREfm, which has since been the predominant species in our hospital. VRE appears to be in an evolutionary flux in our hospital.