Effect of Human Burn Wound Exudate on Pseudomonas aeruginosa Virulence.
Effect of Human Burn Wound Exudate on Pseudomonas aeruginosa Virulence.
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DOI：
10.1128/msphere.00111-15
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发表时间：
2016-03
期刊：
影响因子：
4.8
通讯作者：
Perron K
中科院分区：
文献类型：
--
作者：
Gonzalez MR;Fleuchot B;Lauciello L;Jafari P;Applegate LA;Raffoul W;Que YA;Perron K
严重烧伤创面的微生物感染是目前医学上的一大挑战。在能够定植于此类损伤的细菌的感染中，铜绿假单胞菌的感染是最严重的，导致烧伤患者恢复的重大延迟或导致致命问题。在这项研究中，我们调查了几种烧伤创面病原体在人体烧伤创面分泌的生物液中的生长特性。我们发现，铜绿假单胞菌菌株能够增殖，但不是那些测试的其他病原体。此外，烧伤创面渗出物（BWE）刺激铜绿假单胞菌中毒力因子的表达。BWEs的化学成分分析使我们能够确定这些流体的主要成分。这些数据是必不可少的模拟烧伤创面环境的人工介质的发展和烧伤创面感染的发展中的初始步骤的体外分析。烧伤创面脓毒症是目前烧伤后发病和死亡的主要原因。铜绿假单胞菌、金黄色葡萄球菌和鲍曼不动杆菌等臭名昭著的病原体感染会损害患者的康复，甚至可能导致死亡。在这项研究中，我们调查了烧伤创面渗出物（BWEs）对这些病原体的毒力的影响。收集5例烧伤患者伤后7天内的BWEs。我们首先监测了它们对病原体生长的影响。与鲍曼不动杆菌和金黄色葡萄球菌相比，铜绿假单胞菌是唯一能够在这些人体体液中生长的病原体。与标准实验室培养基相比，典型毒力因子如绿脓菌素和绿脓菌荧光素的表达甚至增强。对斗轮挖掘机进行了详细的化学成分分析，这使我们能够确定斗轮挖掘机的主要成分，并强调烧伤创伤引起的代谢变化。这些数据是必不可少的模拟烧伤创面环境的人工介质的发展和建立一个体外系统来分析烧伤创面感染的初始步骤。严重烧伤创面的微生物感染目前是一个主要的医学挑战。在能够定植于此类损伤的细菌的感染中，铜绿假单胞菌的感染是最严重的，导致烧伤患者恢复的重大延迟或导致致命问题。在这项研究中，我们调查了几种烧伤创面病原体在人体烧伤创面分泌的生物液中的生长特性。我们发现，铜绿假单胞菌菌株能够增殖，但不是那些测试的其他病原体。此外，烧伤创面渗出物（BWE）刺激铜绿假单胞菌中毒力因子的表达。BWEs的化学成分分析使我们能够确定这些流体的主要成分。这些数据是必不可少的模拟烧伤创面环境的人工介质的发展和烧伤创面感染的发展中的初始步骤的体外分析。
Microbial infection of severe burn wounds is currently a major medical challenge. Of the infections by bacteria able to colonize such injuries, those by Pseudomonas aeruginosa are among the most severe, causing major delays in burn patient recovery or leading to fatal issues. In this study, we investigated the growth properties of several burn wound pathogens in biological fluids secreted from human burn wounds. We found that P. aeruginosa strains were able to proliferate but not those of the other pathogens tested. In addition, burn wound exudates (BWEs) stimulate the expression of virulence factors in P. aeruginosa. The chemical composition analysis of BWEs enabled us to determine the major components of these fluids. These data are essential for the development of an artificial medium mimicking the burn wound environment and for in vitro analysis of the initial step in the development of burn wound infections. Burn wound sepsis is currently the main cause of morbidity and mortality after burn trauma. Infections by notorious pathogens such as Pseudomonas aeruginosa, Staphylococcus aureus, and Acinetobacter baumannii impair patient recovery and can even lead to fatality. In this study, we investigated the effect of burn wound exudates (BWEs) on the virulence of those pathogens. BWEs were collected within 7 days after burn trauma from 5 burn patients. We first monitored their effect on pathogen growth. In contrast to A. baumannii and S. aureus, P. aeruginosa was the only pathogen able to grow within these human fluids. Expression of typical virulence factors such as pyocyanin and pyoverdine was even enhanced compared the levels seen with standard laboratory medium. A detailed chemical composition analysis of BWE was performed, which enabled us to determine the major components of BWE and underline the metabolic modifications induced by burn trauma. These data are essential for the development of an artificial medium mimicking the burn wound environment and the establishment of an in vitro system to analyze the initial steps of burn wound infections. IMPORTANCE Microbial infection of severe burn wounds is currently a major medical challenge. Of the infections by bacteria able to colonize such injuries, those by Pseudomonas aeruginosa are among the most severe, causing major delays in burn patient recovery or leading to fatal issues. In this study, we investigated the growth properties of several burn wound pathogens in biological fluids secreted from human burn wounds. We found that P. aeruginosa strains were able to proliferate but not those of the other pathogens tested. In addition, burn wound exudates (BWEs) stimulate the expression of virulence factors in P. aeruginosa. The chemical composition analysis of BWEs enabled us to determine the major components of these fluids. These data are essential for the development of an artificial medium mimicking the burn wound environment and for in vitro analysis of the initial step in the development of burn wound infections.