Role of whole bone marrow, whole bone marrow cultured cells, and mesenchymal stem cells in chronic wound healing.
Role of whole bone marrow, whole bone marrow cultured cells, and mesenchymal stem cells in chronic wound healing.
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DOI：
10.1186/s13287-015-0001-9
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发表时间：
2015-03-13
影响因子：
7.5
通讯作者：
Van Badiavas E
中科院分区：
文献类型：
--
作者：
Rodriguez-Menocal L;Shareef S;Salgado M;Shabbir A;Van Badiavas E
最近的证据表明，骨髓细胞在皮肤伤口愈合的炎症、增殖和重塑阶段发挥关键作用。在递送到伤口的骨髓细胞中有干细胞，其可以分化成多种组织形成细胞谱系以实现愈合。深入了解哪些谱系在加速伤口愈合方面最重要，对于设计难以愈合伤口的治疗方法非常有价值。在这份报告中，我们比较了不同的骨髓制备物对体外伤口愈合试验的影响。检查的制备物是全骨髓（WBM）、全骨髓（基于长期起始/造血的）培养细胞（BMC）和骨髓衍生的间充质干细胞（BM-MSC）。我们还将这些骨髓制剂应用于两种辐射诱导的伤口愈合延迟的小鼠模型中，以确定哪种对愈合具有更大的影响。血管生成试验表明，管形成刺激WBM和BMC，WBM具有最大的效果。划痕试验显示，与BM-MSC相比，WBM存在下在24、48和72小时时的成纤维细胞迁移更高。在辐射诱导的延迟伤口愈合动物模型中，WBM似乎也比BMC和BM-MSC刺激更大的愈合反应。这些研究有望帮助阐明干细胞在慢性伤口修复过程中的作用，并揭示骨髓中存在的哪些细胞可能对伤口愈合过程贡献最大。
Recent evidence has shown that bone marrow cells play critical roles during the inflammatory, proliferative and remodeling phases of cutaneous wound healing. Among the bone marrow cells delivered to wounds are stem cells, which can differentiate into multiple tissue-forming cell lineages to effect, healing. Gaining insight into which lineages are most important in accelerating wound healing would be quite valuable in designing therapeutic approaches for difficult to heal wounds. In this report we compared the effect of different bone marrow preparations on established in vitro wound healing assays. The preparations examined were whole bone marrow (WBM), whole bone marrow (long term initiating/hematopoietic based) cultured cells (BMC), and bone marrow derived mesenchymal stem cells (BM-MSC). We also applied these bone marrow preparations in two murine models of radiation induced delayed wound healing to determine which had a greater effect on healing. Angiogenesis assays demonstrated that tube formation was stimulated by both WBM and BMC, with WBM having the greatest effect. Scratch wound assays showed higher fibroblast migration at 24, 48, and 72 hours in presence of WBM as compared to BM-MSC. WBM also appeared to stimulate a greater healing response than BMC and BM-MSC in a radiation induced delayed wound healing animal model. These studies promise to help elucidate the role of stem cells during repair of chronic wounds and reveal which cells present in bone marrow might contribute most to the wound healing process.
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DOI：
10.1111/wrr.12142
发表时间：
2014-03
期刊：
Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society
影响因子：
--
作者：
Stojadinovic O;Pastar I;Nusbaum AG;Vukelic S;Krzyzanowska A;Tomic-Canic M
通讯作者：
Tomic-Canic M
影响因子：
2.9
作者：
Badiavas, Evangelos V.;Ford, Dwayne;Maizel, Abby
通讯作者：
Maizel, Abby
影响因子：
15.9
作者：
Gallagher, Katherine A.;Liu, Zhao-Jun;Velazquez, Omaida C.
通讯作者：
Velazquez, Omaida C.
影响因子：
3.1
作者：
Harding, Keith G;Moore, Keith;Phillips, Tania J
通讯作者：
Phillips, Tania J
影响因子：
2.3
作者：
Badiavas, EV
通讯作者：
Badiavas, EV