SINGLE-PARTICLE TRACKING - ANALYSIS OF DIFFUSION AND FLOW IN 2-DIMENSIONAL SYSTEMS
SINGLE-PARTICLE TRACKING - ANALYSIS OF DIFFUSION AND FLOW IN 2-DIMENSIONAL SYSTEMS
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DOI：
10.1016/s0006-3495(91)82125-7
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发表时间：
1991-10-01
影响因子：
3.4
通讯作者：
ELSON, EL
中科院分区：
文献类型：
--
作者：
Qian, H;SHEETZ, MP;ELSON, EL
使用视频增强对比显微镜在高空间和时间分辨率下分析小颗粒的轨迹提供了一种强有力的方法来表征活细胞和其他系统中颗粒运动的机制。我们在这里提出的理论基础进行随机扩散和/或在二维系统中的均匀速度系统传输的粒子的这些轨迹的分析。单粒子跟踪方法，基于观察单个粒子的轨迹，与表征大量粒子的运动的方法进行比较，如荧光光漂白恢复。讨论了从粒子的位置或速度的相关性确定扩散系数或输运速度。具有实际意义的结果是分析这些测定的预期统计不确定性对位置测量次数的依赖性。这提供了一种判断使用这种方法获得的扩散系数和输运速度的准确性的方法。
Analysis of the trajectories of small particles at high spatial and temporal resolution using video enhanced contrast microscopy provides a powerful approach to characterizing the mechanisms of particle motion in living cells and in other systems. We present here the theoretical basis for the analysis of these trajectories for particles undergoing random diffusion and/or systematic transport at uniform velocity in two-dimensional systems. The single particle tracking method, based on observations of the trajectories of individual particles, is compared with methods that characterize the motions of a large collection of particles such as fluorescence photobleaching recovery. Determination of diffusion coefficients or transport velocities either from correlation of positions or of velocities of the particles is discussed. A result of practical importance is an analysis of the dependence of the expected statistical uncertainty of these determinations on the number of position measurements. This provides a way of judging the accuracy of the diffusion coefficients and transport velocities obtained using this approach.