Complete mitochondrial genome of the soybean leaffolder, Omiodes indicata (Lepidoptera: Pyraloidea: Crambidae), and phylogenetic analysis for Pyraloidea
Complete mitochondrial genome of the soybean leaffolder, Omiodes indicata (Lepidoptera: Pyraloidea: Crambidae), and phylogenetic analysis for Pyraloidea
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大豆纵叶螟 Omiodes indicata（鳞翅目：螟总科：螟科）的完整线粒体基因组，以及螟总科的系统发育分析
DOI：
10.1016/j.ijbiomac.2018.03.041
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发表时间：
2018-08-01
影响因子：
8.2
通讯作者：
Liu, Xiaomeng
中科院分区：
文献类型：
--
作者：
Yang, Mingsheng;Song, Lu;Liu, Xiaomeng
对印度拟步行虫的线粒体全基因组进行了测序和鉴定。环状有丝分裂基因组全长15, 367 bp，包括13个蛋白质编码基因（PCG）、2个核糖体RNA基因（rRNA）、22个转运RNA基因（tRNA）和一个富含A + T的区域。核苷酸组成高度偏向A + T核苷酸（81.6%）。除了cox 1以CGA起始外，所有13个PCG均以典型起始密码子（ATN）起始。所有的tRNA都具有典型的三叶草结构，除了trnS 1（AGN），其中二氢尿苷（DHU）臂的碱基对减少。号手术indicata中，atp 8和atp 6、trnS 2和nad 1之间的基序“ATGATAA”和“ATACTAA”，以及A + T富集区的基序“ATAG”和“ATTTA”。基于4个数据集的系统发育比较分析表明，包含13个PCG的所有编码位置的数据集在解决更高的系统发育问题上表现出最高的信息量。贝叶斯推理（BI）和最大似然（ML）分析产生普遍支持的系统发育关系之间的11个pyraloid亚科。然而，在ML分析中，Acentropinae、Crambinae、Glaphyriinae、Schoenobiinae和Scopariinae这5个亚科之间的关系是模糊的，这可能在未来基于有丝分裂基因组的系统发育研究中通过大量采样来解决。(C)2018由Elsevier B. V.出版
Complete mitochondrial genome (mitogenome) of the Omiodes indicata was sequenced and characterized. The circular mitogenome is 15,367bp long, including 13 protein-coding genes (PCGs), two ribosomal RNA genes (rRNAs), 22 transfer RNA genes (tRNAs), and an A + T-rich region. Nucleotide composition is highly biased toward A + T nucleotides (81.6%). All 13 PCGs initiate with canonical start codon (ATN), except for cox1 that initiates with CGA. All tRNAs have a typical clover-leaf structure, except for trnS1 (AGN) in which the base pairs of the dihydrouridine (DHU) arm are reduced. In O. indicata, the motifs "ATGATAA" and "ATACTAA" between atp8 and atp6, trnS2 and nad1, respectively, and the motifs "ATAG" and "ATTTA" in the A + T-rich region can be identified. Comparative phylogenetic analyses based on four datasets show that the dataset including all coding positions of 13 PCGs exhibit the highest informativeness in resolving higher phylogeny of Pyraloidea. Bayesian inference (BI) and maximum likelihood (ML) analyses yield generally well-supported phylogenetic relationships among the eleven pyraloid subfamilies involved. However, the relationships among the five subfamilies (Acentropinae, Crambinae, Glaphyriinae, Schoenobiinae and Scopariinae) in ML analysis are ambiguous, which might be resolved by ample sampling in future mitogenome-based phylogenetic studies. (C) 2018 Published by Elsevier B.V.