A non-tandem CCCH-type zinc-finger protein, IbC3H18, functions as a nuclear transcriptional activator and enhances abiotic stress tolerance in sweet potato
A non-tandem CCCH-type zinc-finger protein, IbC3H18, functions as a nuclear transcriptional activator and enhances abiotic stress tolerance in sweet potato
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非串联CCCH型锌指蛋白IbC3H18作为核转录激活剂发挥作用并增强甘薯的非生物胁迫耐受性
DOI：
10.1111/nph.15925
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发表时间：
2019-09-01
期刊：
影响因子：
9.4
通讯作者：
He, Shaozhen
中科院分区：
文献类型：
--
作者：
Zhang, Huan;Gao, Xiaoru;He, Shaozhen
CCCH型锌指蛋白在调节植物生长发育和逆境应答中起着重要作用。然而，非串联CCCH型锌指（非TZF）蛋白的分子和功能特性的特点很少在植物中。在这里，我们报告了甘薯非TZF基因IbC3H18的生物学和分子特征。我们发现IbC3H18具有组织和非生物胁迫特异性表达，并且可以有效地被非生物胁迫诱导，包括NaCl，聚乙二醇（PEG）6000，H2O2和脱落酸（阿坝）在甘薯中。因此，过量表达IbC3H18导致甘薯对盐、干旱和氧化胁迫的耐受性增加，而敲低IbC3H18导致甘薯对盐、干旱和氧化胁迫的耐受性降低。此外，IbC3H18作为核转录激活因子发挥作用，并调节一系列参与活性氧（ROS）清除、阿坝信号传导、光合作用和离子转运途径的非生物胁迫响应基因的表达。此外，我们的数据表明，IbC3H18与IbPR5物理相互作用，并且IbPR5的过表达增强了转基因烟草植物的耐盐性和耐旱性。总的来说，我们的数据表明IbC3H18在提高甘薯的非生物胁迫耐受性中起作用，其可以作为用于提高作物的非生物胁迫抗性的候选基因。
CCCH-type zinc-finger proteins play essential roles in regulating plant development and stress responses. However, the molecular and functional properties of non-tandem CCCH-type zinc-finger (non-TZF) proteins have been rarely characterized in plants. Here, we report the biological and molecular characterization of a sweet potato non-TZF gene, IbC3H18. We show that IbC3H18 exhibits tissue- and abiotic stress-specific expression, and could be effectively induced by abiotic stresses, including NaCl, polyethylene glycol (PEG) 6000, H2O2 and abscisic acid (ABA) in sweet potato. Accordingly, overexpression of IbC3H18 led to increased, whereas knock-down of IbC3H18 resulted in decreased tolerance of sweet potato to salt, drought and oxidation stresses. In addition, IbC3H18 functions as a nuclear transcriptional activator and regulates the expression of a range of abiotic stress-responsive genes involved in reactive oxygen species (ROS) scavenging, ABA signaling, photosynthesis and ion transport pathways. Moreover, our data demonstrate that IbC3H18 physically interacts with IbPR5, and that overexpression of IbPR5 enhances salt and drought tolerance in transgenic tobacco plants. Collectively, our data indicate that IbC3H18 functions in enhancing abiotic stress tolerance in sweet potato, which may serve as a candidate gene for use in improving abiotic stress resistance in crops.