Evidence that disinhibition is associated with a decrease in number of vesicles available for release at inhibitory synapses
Evidence that disinhibition is associated with a decrease in number of vesicles available for release at inhibitory synapses
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DOI：
10.1523/jneurosci.3489-04.2005
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发表时间：
2005-01-26
影响因子：
5.3
通讯作者：
Woolley, CS
中科院分区：
文献类型：
--
作者：
Ledoux, VA;Woolley, CS
我们使用连续电子显微照片的三维重建来研究可能是雌激素诱导的海马 CA1 锥体细胞去抑制的两种结构变化：每个神经元抑制输入数量的减少和/或可能限制 GABA 释放的抑制按钮的变化。我们分析了雌激素处理和对照动物中形成 510 个抑制性突触的 373 个按钮。我们的结果表明，雌激素特异性地减少与抑制性突触突触前膜相邻的突触小泡的数量，而不影响小泡的总数。我们检测到抑制输入的密度没有差异。这些发现为雌激素对突触抑制的功能影响提供了一种新的机制，并代表了第一个体内证据，表明可释放的突触前囊泡的数量是影响突触生理学的突触的调节特性。
We used three- dimensional reconstruction from serial electron micrographs to investigate two structural changes that could underlie estrogen- induced disinhibition of hippocampal CA1 pyramidal cells: a decrease in the number of inhibitory inputs per neuron and/ or a change in inhibitory boutons that could limit GABA release. We analyzed 373 boutons forming 510 inhibitory synapses in estrogen-treated and control animals. Our results show that estrogen specifically decreases the number of synaptic vesicles adjacent to the presynaptic membrane of inhibitory synapses without affecting the overall number of vesicles. We detected no difference in the density of inhibitory inputs. These findings provide a novel mechanism for the functional effects of estrogen on synaptic inhibition and represent the first in vivo evidence that the number of presynaptic vesicles available for release is a regulated property of synapses that affects synaptic physiology.