Kinetics of serotonin uptake in the dog lung.
Kinetics of serotonin uptake in the dog lung.
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狗肺中血清素摄取的动力学。
DOI：
10.1152/jappl.1981.51.2.405
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Dawson,CA
中科院分区：
文献类型：
--
作者：
Rickaby,DA;Linehan,JH;Bronikowski,TA;Dawson,CA
将含有[3 H] 5-羟色胺和吲哚菁绿色染料的大丸剂快速引入离体血液灌注的犬左下肺叶的肺动脉中。进样后约25 s内连续采集静脉流出液样品。注射的[3 H] 5-羟色胺的肺摄入量与注射剂量成反比，范围从10-nmol注射的约70%至100-nmol注射的约38%。在采样间隔内静脉流出液中出现的注射3 H中，小于2%是[3 H]5-羟基吲哚乙酸的形式;在通过肺叶的大剂量转运期间，血小板对5-羟色胺的摄取可以忽略不计。5-羟色胺提取率曲线呈现出剂量依赖性的特征形状，我们使用一个模型进行了解释，该模型假设5-羟色胺摄取遵循Michaelis-Menten动力学，并且通过毛细血管床的流动是不均匀的。使用该模型和多元回归分析，使用流出染料和3 H浓度与时间的曲线来估计肺摄取5-羟色胺的Km和Vmax。
A bolous containing [3H]serotonin and indocyanine green dye was rapidly introduced into the pulmonary artery on an isolated blood-perfused left lower dog lung lobe. Sequential samples of the venous effluent were collected for approximately 25 s following the injections. The lung intake of the injected [3H]serotonin was inversely proportional to the injected dose, ranging from about 70% for a 10-nmol injection to about 38% for a 100-nmol injection. Less than 2% of the injected 3H, which appeared in the venous effluent over the sampling interval, was in the form of [3H]5-hydroxyindoleacetic acid; and during the bolous transit through the lung lobe, uptake of serotonin by platelets was negligible. The serotonin extraction ratio curves exhibited dose-dependent characteristic shapes that we have interpreted using a model in which it is assumed that serotonin uptake follows Michaelis-Menten kinetics and flow through the capillary bed is heterogeneous. Using this model and a multiple regression analysis, the outflow dye and 3H concentration vs. time curves were used to estimate Km and Vmax for serotonin uptake by the lungs.