Engineering Cyanobacteria To Synthesize and Export Hydrophilic Products
Engineering Cyanobacteria To Synthesize and Export Hydrophilic Products
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DOI：
10.1128/aem.00202-10
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发表时间：
2010-06-01
影响因子：
4.4
通讯作者：
Way, Jeffrey C.
中科院分区：
文献类型：
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作者：
Niederholtmeyer, Henrike;Wolfstaedter, Bernd T.;Way, Jeffrey C.
蓝藻代谢工程的优点是阳光和CO(2) 是这些生物体的唯一能量和碳源。然而，作为光合自养生物，蓝藻通常缺乏转运蛋白来跨膜移动亲水性初级代谢物。为了解决蓝藻是否可以被改造为产生和分泌有机初级代谢物，细长聚球藻 PCC7942 被改造为表达编码转化酶和葡萄糖促进剂的基因，从而介导葡萄糖和果糖的分泌。类似地，乳酸脱氢酶和乳酸转运蛋白编码基因的表达允许乳酸在细胞外介质中积累。相关转运蛋白的表达对于分泌至关重要。通过表达额外的异源酶进一步提高了这些分子的产量。工程蓝细菌分泌的糖可用于在缺乏额外营养源的情况下支持大肠杆菌生长。这些结果表明蓝细菌可以被改造来生产和分泌高价值的亲水产物。
Metabolic engineering of cyanobacteria has the advantage that sunlight and CO(2) are the sole source of energy and carbon for these organisms. However, as photoautotrophs, cyanobacteria generally lack transporters to move hydrophilic primary metabolites across membranes. To address whether cyanobacteria could be engineered to produce and secrete organic primary metabolites, Synechococcus elongatus PCC7942 was engineered to express genes encoding an invertase and a glucose facilitator, which mediated secretion of glucose and fructose. Similarly, expression of lactate dehydrogenase- and lactate transporter-encoding genes allowed lactate accumulation in the extracellular medium. Expression of the relevant transporter was essential for secretion. Production of these molecules was further improved by expression of additional heterologous enzymes. Sugars secreted by the engineered cyanobacteria could be used to support Escherichia coli growth in the absence of additional nutrient sources. These results indicate that cyanobacteria can be engineered to produce and secrete high-value hydrophilic products.