Gly-X-Y tripeptide frequencies in collagen: A context for host-guest triple-helical peptides
Gly-X-Y tripeptide frequencies in collagen: A context for host-guest triple-helical peptides
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DOI：
10.1006/jsbi.1998.3977
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发表时间：
1998-01-01
影响因子：
3
通讯作者：
Brodsky, B
中科院分区：
文献类型：
--
作者：
Ramshaw, JAM;Shah, NK;Brodsky, B
胶原蛋白三螺旋由重复的 (Gly-X-Y)(n) 序列组成。理论上，有超过 400 种可能的 Gly-X-Y 三联体，但对纤维状和非纤维状胶原蛋白序列的分析表明，仅发现了数量有限的三联体，而且许多三联体从未被观察到。Gly-X-Y 三联体的非随机频率使得通过研究一组有限的主客体肽来实验性地接近大部分胶原蛋白序列的稳定性变得可行。在这些肽中，单个 Gly-X-Y三联体构成客体，而宿主由 Gly-Pro-Hyp 三肽组成。一组主客体肽被设计为含有胶原蛋白中最常见的非极性和带电三联体。所有这些都形成了稳定的三螺旋，其熔化温度取决于客体三联体的身份。虽然包含所有可能的三联体中不到 10%，但该数据集涵盖了 50-60% 的胶原蛋白序列，并为建立稳定性量表提供了一个起点，以预测重要胶原蛋白区域（例如基质金属蛋白酶切割位点或结合位点）的相对稳定性。 (C) 1998 年学术出版社。
The collagen triple-helix consists of a repeating (Gly-X-Y)(n) sequence. In theory, there are more than 400 possible Gly-X-Y triplets, but analysis of sequences from fibrillar and nonfibrillar collagens shows that only a limited set of triplets ape found in significant numbers, and many are never observed, The nonrandom frequency of Gly-X-Y triplets makes it practical to experimentally approach the stability of much of the collagen sequence through the study of a Limited set of host-guest peptides, In these peptides, individual Gly-X-Y triplets constitute the guest, while the host consists of Gly-Pro-Hyp tripeptides. A set of host-guest peptides was designed to contain the most common nonpolar and charged triplets found in collagen. All formed stable triple-helices, with their melting temperature depending on the identity of the guest triplet. While including less than 10% of all possible triplets, the data set covers 50-60% of collagen sequences and provides a starting point for establishing a stability scale to predict the relative stability of important collagen regions, such as the matrix metalloproteinase cleavage site or binding sites. (C) 1998 Academic Press.