Knockdown of CD146 reduces the migration and proliferation of human endothelial cells
Knockdown of CD146 reduces the migration and proliferation of human endothelial cells
复制标题
DOI：
10.1038/sj.cr.7310039
复制
发表时间：
2006-03-01
期刊：
影响因子：
44.1
通讯作者：
Yan, Xiyun
中科院分区：
文献类型：
--
作者：
Kang, Yanyong;Wang, Fengcai;Yan, Xiyun
我们前期的研究表明cd146分子是血管内皮的生物标志物，参与血管生成和肿瘤生长。然而，背后的机制尚不清楚。本研究首次利用cd146 siRNA开发了一种新的cd146阻断系统，研究其对内皮细胞的作用。我们的数据显示，CD146 siRNA在mRNA和蛋白水平上特异性阻断CD146的表达，导致HUVEC的增殖、粘附和迁移受到显著抑制。这些结果表明，CD146在血管内皮细胞活性和血管生成中起着关键作用，CD146 siRNA可以作为抗血管生成治疗的新抑制剂。
Our previous study has demonstrated that CD 146 molecule is a biomarker on vascular endothelium, which is involved in angiogenesis and tumor growth. However the mechanism behind is not clear. Here we have for the first time developed a novel CD 146 blockade system using CD 146 siRNA to study its function on endothelial cells. Our data showed that CD146 siRNA specifically blocked the expression of CD146 on both mRNA and protein levels, leading to the significant suppression of HUVEC proliferation, adhesion and migration. These results demonstrate that CD146 plays a key role in vascular endothelial cell activity and angiogenesis, and CD146 siRNA can be used as a new inhibitor for anti-angiogenesis therapy.