AN ALKYL HYDROPEROXIDE REDUCTASE INDUCED BY OXIDATIVE STRESS IN SALMONELLA-TYPHIMURIUM AND ESCHERICHIA-COLI - GENETIC-CHARACTERIZATION AND CLONING OF AHP
AN ALKYL HYDROPEROXIDE REDUCTASE INDUCED BY OXIDATIVE STRESS IN SALMONELLA-TYPHIMURIUM AND ESCHERICHIA-COLI - GENETIC-CHARACTERIZATION AND CLONING OF AHP
复制标题
DOI：
10.1128/jb.171.4.2049-2055.1989
复制
发表时间：
1989-04-01
影响因子：
3.2
通讯作者：
AMES, BN
中科院分区：
文献类型：
--
作者：
STORZ, G;JACOBSON, FS;AMES, BN
从鼠伤寒沙门氏菌和大肠杆菌中定位并克隆了编码组成烷基氢过氧化物还原酶的两种蛋白质（F52 a和C22）的ahp基因。分离出两类过表达这两种蛋白质的抗氧化剂ahp突变体。ahp-1是在野生型背景下分离的，并且依赖于oxyR，oxyR是一种抗氧化应激防御的正调节剂。ahp-2是在oxyR缺失背景下分离的，并且不依赖于oxyR。与ahp-1和ahp-2连锁或插入ahp中的转座子将基因定位在S.在两株鼠伤寒沙门氏菌中均发现ahp基因缺失，59%与内毒素连锁。菌和大肠大肠杆菌导致对氢过氧化枯烯（一种烷基氢过氧化物）的杀伤超敏反应，并从二维凝胶和免疫印迹中消除蛋白质F52 a和C22。ahp克隆从两个S.菌和大肠大肠杆菌补充了ahp缺失菌株的氢过氧化枯烯敏感性，并恢复了F52 a和C22蛋白的表达。在S.鼠伤寒沙门氏菌，而不是E. coli ahp基因座。
The ahp genes encoding the two proteins (F52a and C22) that make up an alkyl hydroperoxide reductase were mapped and cloned from Salmonella typhimurium and Escherichia coli. Two classes of oxidant-resistant ahp mutants which overexpress the two proteins were isolated. ahp-1 was isolated in a wild-type background and is dependent on oxyR, a positive regulator of defenses against oxidative stress. ahp-2 was isolated in an oxyR deletion background and is oxyR independent. Transposons linked to ahp-1 and ahp-2 or inserted in ahp mapped the genes to 13 min on the S. typhimurium chromosome, 59% linked to ent. Deletions of ahp obtained in both S. typhimurium and E. coli resulted in hypersensitivity to killing by cumene hydroperoxide (an alky hydroperoxide) and elimination of the proteins F52a and C22 from two-dimensional gels and immunoblots. ahp clones isolated from both S. typhimurium and E. coli complemented the cumene hydroperoxide sensitivity of the ahp deletion strains and restored expression of the F52a and C22 proteins. A cis-acting element required for oxyR-dependent, rpoH-independent heat shock induction of the F52a protein was present at the S. typhimurium but not the E. coli ahp locus.