Cyclic GMP accumulation during cholinergic stimulation of eccrine sweat glands.
Cyclic GMP accumulation during cholinergic stimulation of eccrine sweat glands.
复制标题
小汗腺胆碱能刺激期间循环 GMP 积累。
DOI：
10.1152/ajpcell.1984.247.3.c234
复制
发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Sato,F
中科院分区：
文献类型：
--
作者：
Sato,K;Sato,F
本文利用离体猴掌分泌汗腺，比较了鸟苷3′5′-环单磷酸鸟苷（cGMP）积累时间与汗液分泌的关系，探讨了cGMP可能是细胞内胆碱能刺激[甲基氯胆碱（MCh）]的介质。分离的汗腺与MCh或其他试剂孵育，用放射免疫法测定组织中cGMP的水平。平行实验中，体外培养单个汗腺诱导汗液分泌。MCh刺激使cGMP水平瞬时升高，从10 fmol/腺体升高到80 fmol/腺体，在1-2分钟达到峰值，但在5分钟后恢复到基础水平。mch诱导的cGMP水平呈剂量依赖性，可被阿托品抑制。然而，离子载体A23187 （2 × 10(-4) M）导致cGMP水平持续升高至少20分钟。与MCh刺激相比，10(-4)M MNNG和8-溴-cGMP （2 mM）均未诱导汗液分泌。因此，尽管cGMP水平和出汗速率之间的平行关系似乎适用于mch诱导的出汗的初始阶段，但并不适用于汗液分泌的稳定状态。数据不能被解释为支持cGMP可能是胆碱能汗液分泌的细胞内介质的观点。
The possibility that guanosine 3'5'-cyclic monophosphate (cGMP) may be an intracellular mediator of cholinergic stimulation [methacholine chloride (MCh)] was explored by comparing the relationship between the time course of cGMP accumulation and sweat secretion by use of isolated monkey palm eccrine sweat glands. Isolated sweat glands were incubated with MCh or other agents, and tissue levels of cGMP were determined by radioimmunoassay. In parallel experiments, sweat secretion was induced from cannulated single sweat glands in vitro. Stimulation with MCh produced a Ca-dependent transient elevation of cGMP level from 10 to 80 fmol/gland, peaking at 1-2 min but returning to the basal level by 5 min. The MCh-induced cGMP level was dose dependent and was inhibited by atropine. Ionophore A23187 (2 X 10(-4) M), however, caused persistent elevation of cGMP level for at least 20 min. Neither 10(-4) M MNNG, which elevated the cGMP level comparably with MCh stimulation, nor 8-bromo-cGMP (2 mM) induced sweat secretion. Thus although a parallelism between the cGMP level and sweating rate appears to hold for the initial stage of MCh-induced sweating, it does not hold for the steady state of sweat secretion. Data could not be interpreted to favor the notion that cGMP may be the intracellular mediator of cholinergic sweat secretion.
登录
查看更多内容
意识、个人身份和分裂的大脑
影响因子：
2.6
作者：
R. Sperry
通讯作者：
R. Sperry
发育性失语症：作为感觉方式函数的非语言处理率受损。
影响因子：
2.6
作者：
P. Tallal;M. Piercy
通讯作者：
M. Piercy
认知功能的不对称作为职业咨询和人员分类的可能预测因素
DOI：
--
发表时间：
1983
期刊：
影响因子：
--
作者：
Itzhak Harpaz
通讯作者：
Itzhak Harpaz
顶叶损伤对空间可逆操作性能的影响。
影响因子：
2.6
作者：
Nelson Butters;Melvin Barton
通讯作者：
Melvin Barton
男性脑连合切开术
DOI：
--
发表时间：
1965
期刊：
影响因子：
--
作者：
J. Bogen;M. Gazzaniga
通讯作者：
M. Gazzaniga