Distribution of immunoreactive von Willebrand factor in the microvascular network of bovine cystic follicles
Distribution of immunoreactive von Willebrand factor in the microvascular network of bovine cystic follicles
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牛囊性滤泡微血管网络中免疫反应性血管性血友病因子的分布
DOI：
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发表时间：
2002
期刊：
影响因子：
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通讯作者：
T. Nakao
中科院分区：
文献类型：
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作者：
N. Isobe;Y. Yoshimura;T. Nakao
本研究旨在探讨囊性卵泡微血管中von Willebrand因子（vWF）的表达是否与闭锁卵泡中的表达不同。用兔抗vWF单克隆抗体对健康、闭锁和囊性卵泡石蜡切片进行免疫染色。vwf阳性细胞分布在卵泡的四个不同区域，从卵泡顶端到卵泡基部。在所有类型的卵泡中，vWF在毛细血管内皮细胞以及卵膜内外静脉和动脉中观察到免疫反应。在卵泡内，与晚期和晚期闭锁卵泡相比，A型和B型卵泡的vwf阳性区域明显减少。外膜各类型囊泡vwf阳性血管及vwf阳性面积均明显小于健康或闭锁卵泡。这些结果提示，在囊泡中，vWF在卵泡微血管系统的诱导减少，可能抑制了血管系统的退化。脉管系统的持续稳定可能是延迟囊泡组织退化的因素之一，也有助于源自血清的卵泡液的积累。
The present study was undertaken to examine whether the von Willebrand factor (vWF) expression in the follicular microvasculature in the cystic follicles differs from that in the atretic follicles. Paraffin sections of healthy, atretic and cystic follicles were immunostained with rabbit monoclonal antibody to vWF. The vWF-positive cells were counted in four different regions of a follicle from the apical to the basal side. In all types of follicles, immunoreactions for vWF were observed in the endothelial cells of capillaries as well as veins and arteries in the theca interna and externa. In the theca interna, vWF-positive areas were significantly lower in the Type A and B cystic follicles compared to advanced and late atretic follicles. In the theca externa, the vWF-positive blood vessels and vWF-positive area were significantly smaller in all types of cystic follicles than in the healthy or atretic follicles. From these results, it is suggested that in the cystic follicles the induction of vWF in the follicular microvasculature system is reduced, which may suppress the degeneration of vascular system. Continuation of stability in vasculature may be one of the factors that delays the tissue regression in the cystic follicles, and also contributes to the accumulation of follicular fluid that originates from the serum.
促性腺激素引发的未成熟大鼠排卵期间卵巢血量增加。
影响因子：
3.6
作者：
Tanaka,N;Espey,LL;Okamura,H
通讯作者：
Okamura,H