Autophagy and Reactive Oxygen Species Are Involved in Neutrophil Extracellular Traps Release Induced by C. albicans Morphotypes.
Autophagy and Reactive Oxygen Species Are Involved in Neutrophil Extracellular Traps Release Induced by C. albicans Morphotypes.
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DOI：
10.3389/fmicb.2016.00879
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发表时间：
2016
影响因子：
5.2
通讯作者：
Monari C
中科院分区：
文献类型：
--
作者：
Kenno S;Perito S;Mosci P;Vecchiarelli A;Monari C
中性粒细胞胞外陷阱（NETs）是DNA纤维和颗粒酶（如弹性酶和髓过氧化物酶）的结合。在这项研究中，我们证明了白色念珠菌菌丝（CAH）细胞和酵母（CAY）细胞诱导NET释放的数量、动力学和机制的差异。与CAY细胞相比，CAH细胞诱导的NET数量更多，并且可以在孵育4小时内刺激NET的快速形成。CAY细胞也能够诱导快速NET形成，但这种能力在4小时后就丧失了。活性氧（ROS）和自噬都与CAH和CAY细胞诱导的NET有关，但这两种机制的参与时间不同。特别是，在早期（15分钟）CAH细胞通过自噬刺激NET，但不通过ROS，而CAY细胞通过自噬和ROS诱导NET。在4小时，只有CAH细胞通过自噬和ROS刺激NET的形成。最后，我们证明，在CAH细胞的反应中，NET释放涉及NF-κB活化，并与菌丝破坏密切相关。
Neutrophil extracellular traps (NETs) are a combination of DNA fibers and granular enzymes, such as elastase and myeloperoxidase. In this study, we demonstrate that Candida albicans hyphal (CAH) cells and yeast (CAY) cells induce differential amounts, kinetics and mechanisms of NET release. CAH cells induced larger quantities of NET compared to CAY cells and can stimulate rapid NET formation up to 4 h of incubation. CAY cells are, also, able to induce rapid NET formation, but this ability was lost at 4 h. Both reactive oxygen species (ROS) and autophagy are implicated in NET induced by CAH and CAY cells, but with a time-different participation of these two mechanisms. In particular, in the early phase (15 min) CAH cells stimulate NET via autophagy, but not via ROS, while CAY cells induce NET via both autophagy and ROS. At 4 h, only CAH cells stimulate NET formation using autophagy as well as ROS. Finally, we demonstrate that NET release, in response to CAH cells, involves NF-κB activation and is strongly implicated in hyphal destruction.