Intermediate filaments and their associates: multi-talented structural elements specifying cytoarchitecture and cytodynamics
Intermediate filaments and their associates: multi-talented structural elements specifying cytoarchitecture and cytodynamics
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DOI：
10.1016/s0955-0674(99)00060-5
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发表时间：
2000-02-01
影响因子：
7.5
通讯作者：
Aebi, U
中科院分区：
文献类型：
--
作者：
Herrmann, H;Aebi, U
中间丝（IF）阵列的组装涉及一组复杂的细胞类型特异性IF相关蛋白的募集。它们中的一些是完整的膜蛋白，其他的作为交联蛋白与矢量结合活性，还有其他的包括马达蛋白，在体内IF似乎主要是杂聚物，虽然在体外几个IF蛋白（如波形蛋白，结蛋白，神经丝（NF）-L和核纤层蛋白）做自组装成IF样聚合物。相反，NF-M，NF-H，巢蛋白，synemin和paranemin，所有真正的IF蛋白，都不能在体外或体内自组装成Ifs。这个大的多基因家族的个别IF蛋白是化学异质性的，表现出不同的组装动力学和产生离散类型的细丝。这些独特的IF分子构建块的独特物理性质和相互作用能力与辅助蛋白结合，介导高度动态和互连的细胞类型特异性细胞结构的产生。
The assembly of intermediate filament (IF) arrays involves the recruitment of a complex set of cell-type-specific IF-associated proteins. Some of them are integral membrane proteins, others act as crosslinking proteins with vectorial binding activities, and yet others comprise motor proteins, in vivo IFs appear to be predominantly heteropolymers, although in vitro several IF proteins (e.g. vimentin, desmin, neurofilament (NF)-L and the nuclear lamins) do self-assemble into IF-like polymers. In contrast, NF-M, NF-H, nestin, synemin and paranemin, all bona fide IF proteins, are unable to self-assemble into Ifs either in vitro or in vivo. The individual IF proteins of this large multigene family are chemically heterogeneous, exhibiting different assembly kinetics and yielding discrete types of filaments. The unique physical properties and interaction capabilities of these distinct IF molecular building blocks, in combination with accessory proteins, mediate the generation of a highly dynamic and interconnected, cell-type-specific cytoarchitecture.