Biogenically proficient synthesis and characterization of silver nanoparticles (Ag-NPs) employing aqueous extract of Aquilegia pubiflora along with their in vitro antimicrobial, anti-cancer and other biological applications
Biogenically proficient synthesis and characterization of silver nanoparticles (Ag-NPs) employing aqueous extract of Aquilegia pubiflora along with their in vitro antimicrobial, anti-cancer and other biological applications
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DOI：
10.1016/j.jmrt.2021.08.048
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发表时间：
2021-11-01
影响因子：
6.4
通讯作者：
Abbasi, Bilal Haider
中科院分区：
文献类型：
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作者：
Jan, Hasnain;Zaman, Gouhar;Abbasi, Bilal Haider
在几项研究中，报道了毛花Aquilegia pubiflora叶对抗肝炎，用于治疗皮肤烧伤，伤口愈合，黄疸，妇科，循环和心血管疾病。因此，采用绿色合成法合成纳米银粒子是可行的。毛蕊花叶提取物作为化学合成的替代物，从其生物应用的观点来看是有益的。通过紫外光谱、扫描电子显微镜（SEM）、X射线粉末衍射（XRD）、透射电子显微镜（TEM）、能量色散X射线（EDX）、傅里叶变换红外光谱（FTIR）和高效液相色谱（HPLC）等方法对纳米银粒子的形成和粒径分布进行了表征。粒径为19 nm的银纳米颗粒用于各种测定，包括抗微生物、抗氧化剂、抗阿尔茨海默病、抗糖尿病、抗衰老、抗癌和生物相容性研究。我们的研究结果表明，A.毛花介导的Ag-NP对细菌和真菌菌株都具有高活性，其中大肠杆菌和尼日尔分别显示出最高的抑制区10.9 +/-0.29 mm和13.11 +/-0.33 mm。NP显示出对热带利什曼原虫的剂量依赖性细胞毒活性，其中前鞭毛体的显著IC 50为175 mg/mL，无鞭毛体形式的IC 50为169 μ g/mL。Ag-NP被证明对HepG 2细胞系具有高毒性，并且在Ag-NP处理24小时后抑制细胞活力高达52.06 +/-2.9%。Ag-NPs还显示出对参与阿尔茨海默病的乙酰胆碱酯酶（AChE：71.38 +/-1.75）和丁酰胆碱酯酶（BChE：73.61 +/-1.96）的优异抑制潜力。总的来说，参与衰老、糖尿病和炎症的酶对Ag-NPs显示出适度的抑制反应，从而得出结论，它们是各种生物应用的有希望的选择。(C)2021作者（S）出版社：Elsevier B. V.
Aquilegia pubiflora leaves are reported against hepatitis, for treatment of skin burns, wound healing, jaundice, gynea, circulatory and cardiovascular disease in several studies. Therefore, green synthesis of silver nanoparticles with A. pubiflora leaves extract, as an alternative to chemical synthesis, is beneficial from its biological applications point of view. The formation and size distribution of Ag-NPs were confirmed by UV-spectroscopy, Scanning electron microscopy (SEM), X-ray powder diffraction (XRD), Transmission electron microscopy (TEM), Energy Dispersive X-ray (EDX), Fourier transform infrared spectroscopy (FTIR) and HPLC. Silver nanoparticles with a particle size of 19 nm were used in various assays including antimicrobial, antioxidant, anti-Alzheimer, anti-diabetic, antiaging, anti-cancer and biocompatibility studies. Our findings revealed that A. pubiflora mediated Ag-NPs were highly active against both bacterial and fungal strains with Escherichia coli and Aspergillus niger displaying the highest zone of inhibition 10.9 +/- 0.29 mm and 13.11 +/- 0.33 mm respectively. Dose dependent cytotoxic activity was shown by NPs against Leishmania Tropica with significant IC50 of 175 mg/mL for promastigote and 169 mu g/mL for amastigote forms. The Ag-NPs proved to be highly toxic for the HepG2 cell line and inhibit cell viability up to 52.06 +/- 2.9% after 24 h of Ag-NPs treatment. Ag-NPs also showed excellent inhibitory potential against the enzymes acetylcholinesterase (AChE: 71.38 +/- 1.75) and butyrylcholinesterase (BChE: 73.61 +/- 1.96) which are involved in Alzheimer's disease. Overall, the enzymes involved in aging, diabetes, and inflammation showed a moderate inhibitory response to Ag-NPs, led to the conclusion that they are a promising choice for various biological applications. (C) 2021 The Author(s). Published by Elsevier B.V.