Simultaneous determination of three banned psychiatric drugs in pig feed and tissue using solid-phase reactor on-line oxidizing and HPLC-fluorescence detection.
Simultaneous determination of three banned psychiatric drugs in pig feed and tissue using solid-phase reactor on-line oxidizing and HPLC-fluorescence detection.
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DOI：
10.1002/bmc.3455
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发表时间：
2015-03
期刊：
影响因子：
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通讯作者：
Liang Qi;Li-Min Duan;Xiaoyun Sun;Jing Zhang;Zhiqi Zhang
中科院分区：
文献类型：
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作者：
Liang Qi;Li-Min Duan;Xiaoyun Sun;Jing Zhang;Zhiqi Zhang
禁止在动物饲料和食品中添加吩噻嗪类精神药物，增加了人体器官损伤的风险。吩噻嗪通常表现出弱的天然荧光，并且可以被氧化成强荧光化合物。建立了一种新的、灵敏、简便的二氧化铅固相反应器柱后在线氧化高效液相色谱-荧光检测法，用于同时测定异丙嗪、氯丙嗪和硫利达嗪3种禁用精神药物。采用Agilent TC-C18色谱柱，以乙腈（A）和水（B）（均含0.5%（v/v）甲酸）为移动的流动相，成功地分离了三种化合物。程序梯度洗脱，荧光检测波长分别为：异丙嗪332/373 nm，氯丙嗪340/380 nm，硫利达嗪352/432 nm。异丙嗪、氯丙嗪和硫利达嗪在30.0-4976.4 µg/L、2.0-2153.2 µg/L和15.0-3088.0 µg/L浓度范围内线性良好，相关系数（r）≥0.995。该方法用于猪饲料和猪组织中吩噻嗪类药物的测定，平均加标回收率为69.1- 115.4%。
The banned addition of psychiatric drugs such as phenothiazines to animal feed and foodstuffs increases the risk of human organ lesion. Phenothiazines usually exhibit weak native fluorescence and can be oxidized to strongly fluorescent compounds. In this study, a novel, sensitive and convenient method of HPLC-fluorescence detection based on post-column on-line oxidizing with lead dioxide solid-phase reactor has been developed for simultaneous determination of three banned psychotropic drugs, promethazine, chlorpromazine and thioridazine. Three compounds were successfully separated on an Agilent TC-C18 column with mobile phase of acetonitrile (A) and water (B), both containing 0.5% (v/v) formic acid. A gradient elution was programmed and fluorimetric detection was performed at λex /λem of 332/373 nm for promethazine, 340/380 nm for chlorpromazine and 352/432 nm for thioridazine. The calibration graphs gave good linearity over the concentration ranges of 30.0-4976.4 µg/L for promethazine, 2.0-2153.2 µg/L for chlorpromazine, and 15.0-3088.0 µg/L for thioridazine, and correlation coefficients (r) were ≥0.995. The method was applied to the determination of phenothiazines in pig feed and pig tissue, and the average spiked recoveries were in the range 69.1-115.4%.