B plexins activate Rho through PDZ-RhoGEF
B plexins activate Rho through PDZ-RhoGEF
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DOI：
10.1016/s0014-5793(02)03323-9
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发表时间：
2002-10-09
期刊：
影响因子：
3.5
通讯作者：
Collard, JG
中科院分区：
文献类型：
--
作者：
Driessens, MHE;Olivo, C;Collard, JG
神经丛蛋白是排斥性轴突导向分子信号蛋白的受体。以前，我们已经表明，丛蛋白B1结合激活的Rac，但丛蛋白B1的集群导致Rho激活，导致应力纤维形成。利用酵母双杂交系统，我们发现B丛蛋白的C-末端通过其PDZ结构域直接与Rho特异性交换因子相互作用。丛蛋白-B1的羧基末端氨基酸的突变或显性负PDZ-RhoGEF的共表达废除了丛蛋白-B1引起应力纤维形成的能力。我们的研究结果表明PDZ-RhoGEF在B丛蛋白介导的Rho/Rho激酶信号转导的激活中的作用，涉及轴突导向和细胞迁移的调节。(C)2002年欧洲生物化学学会联合会。由Elsevier Science B. V.出版，版权所有。
Plexins are receptors for the repulsive axon guidance molecules semaphorins. Previously, we have shown that plexin-B1 binds activated Rac, but that clustering of plexin-B1 causes Rho activation, resulting in stress fiber formation. Using the yeast two-hybrid system, we found that the C-terminus of B plexins interacted directly with Rho-specific exchange factors, via their PDZ domain. Mutation of the carboxy-terminal amino acids of plexin-B1 or coexpression of a dominant negative PDZ-RhoGEF abrogated the ability of plexin-B1 to cause stress fiber formation. Our results demonstrate a role for PDZ-RhoGEF in B plexin-mediated activation of Rho/Rho kinase signaling, implicated in the regulation of axon guidance and cell migration. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.