Efficacy of Novel Collagen/Gelatin Scaffold With Sustained Release of Basic Fibroblast Growth Factor for Dermis-like Tissue Regeneration
Efficacy of Novel Collagen/Gelatin Scaffold With Sustained Release of Basic Fibroblast Growth Factor for Dermis-like Tissue Regeneration
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DOI：
10.1097/sap.0b013e318222832f
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发表时间：
2012-11-01
影响因子：
1.5
通讯作者：
Suzuki, Shigehiko
中科院分区：
文献类型：
--
作者：
Kanda, Norikazu;Morimoto, Naoki;Suzuki, Shigehiko
我们已经开发了明胶浓度为10重量%的胶原/明胶海绵（CGS），以维持碱性成纤维细胞生长因子（bFGF）的释放。本研究的目的是阐明浸有不同浓度的bFGF的CGS的功效，使用小鼠皮肤缺损。将浸有生理盐水溶液（NSS）或bFGF溶液（1、7、14或50 μ g/cm（2））的CGS植入小鼠背部的全层皮肤缺损处。对CGS中的伤口面积、新上皮长度和新形成的毛细血管的总面积进行了评价。CGS +7 μ g/cm（2）bFGF组在所有评价项目上均显著上级NSS组。用适当剂量的bFGF浸渍的CGS加速真皮样组织的形成比现有的人工真皮早2或3倍。CGS和bFGF的联合应用可以解决现有人工真皮存在的问题，在皮肤缺损的治疗中具有广阔的应用前景。
We have developed collagen/gelatin sponges (CGS) with a gelatin concentration of 10 wt% to sustain the release of basic fibroblast growth factor (bFGF). The objective of this study is to elucidate the efficacy of CGS impregnated with different concentrations of bFGF, using mouse skin defects. CGSs impregnated with normal saline solution (NSS) or bFGF solution (1, 7, 14, or 50 mu g/cm(2)) were implanted into full-thickness skin defects on the backs of mice. The wound area, neoepithelium length, and total area of newly formed capillaries in CGS were evaluated. The group of CGS with 7-mu g/cm(2) bFGF was significantly superior to the NSS group in all evaluated items. CGS impregnated with the appropriate dosage of bFGF accelerates dermis-like tissue formation 2 or 3 times earlier than existing artificial dermis. The combination of CGS and bFGF could solve the problem of the existing artificial dermis and be very promising for the treatment of skin defects.