Detection of multiple noroviruses associated with an international gastroenteritis outbreak linked to oyster consumption
Detection of multiple noroviruses associated with an international gastroenteritis outbreak linked to oyster consumption
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DOI：
10.1128/jcm.01327-06
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发表时间：
2006-11-01
影响因子：
9.4
通讯作者：
Ruggeri, Franco M.
中科院分区：
文献类型：
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作者：
Le Guyader, Francoise S.;Bon, Fabienne;Ruggeri, Franco M.
使用流行病学和临床数据以及贝类样本分析了与牡蛎消费有关的国际疫情，该疫情涉及意大利的200多人和法国13个较小集群的127名受试者。收集了位于法国南部泻湖的牡蛎产区的环境信息，以调查可能导致污染的事件。使用相同的引物组，通过逆转录-PCR（RT-PCR）对临床和环境样本进行病毒学分析。测序后，通过欧洲食源性病毒科学网络运营的数据库分析数据。实验室之间的国际合作对于快速连接数据和充分解释结果至关重要，因为由于不同病例中涉及的几种诺如病毒株的多样性，一种食物可能是联系并不明显。研究还表明，大雨是造成海产品意外污染的原因，通过实时RT-PCR检测，每个牡蛎的基因组拷贝数高达数百个。
An international outbreak linked to oyster consumption involving a group of over 200 people in Italy and 127 total subjects in 13 smaller clusters in France was analyzed using epidemiological and clinical data and shellfish samples. Environmental information from the oyster-producing area, located in a lagoon in southern France, was collected to investigate the possible events leading to the contamination. Virologic analyses were conducted by reverse transcription-PCR (RT-PCR) using the same primer sets for both clinical and environmental samples. After sequencing, the data were analyzed through the database operated by the scientific network FoodBorne Viruses in Europe. The existence of an international collaboration between laboratories was critical to rapidly connect the data and to fully interpret the results, since it was not obvious that one food could be the link because of the diversity of the several norovirus strains involved in the different cases. It was also demonstrated that heavy rain was responsible for the accidental contamination of seafood, leading to a concentration of up to hundreds of genomic copies per oyster as detected by real-time RT-PCR.