A novel and sensitive resonance scattering assay for detection of urea in serum coupled urease catalytic reaction and NH4+ associated particle reaction
A novel and sensitive resonance scattering assay for detection of urea in serum coupled urease catalytic reaction and NH4+ associated particle reaction
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一种新颖、灵敏的共振散射测定法，用于检测血清中尿素偶联脲酶催化反应和 NH4 相关颗粒反应
DOI：
10.1007/s00449-011-0513-3
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发表时间：
2011-06-01
影响因子：
3.8
通讯作者：
Jiang, Zhiliang
中科院分区：
文献类型：
--
作者：
Liang, Aihui;Qin, Huimin;Jiang, Zhiliang
在pH为6.6的Na2HPO4-NaH2PO4缓冲溶液中，脲酶催化剂存在下，尿素可分解生成NH4（+）。NH4（+）与四苯基硼钠（NaTPB）反应形成缔合粒子，在474 nm处出现共振散射峰。随着尿素浓度的增加，NH4（+）增加，474 nm处RS强度线性增强。在所选条件下，增加RS强度（Delta I (474 nm)）与尿素浓度在0.125 ~ 15 μ M范围内呈线性关系，尿素检出限为0.058 μ M，回归方程为Delta I (474 nm) = 31.6C + 2.1，相关系数为0.9986。该催化RS法用于人血清样品中尿素的检测，具有良好的选择性和灵敏度，结果与参考方法一致。
In the pH 6.6 Na2HPO4-NaH2PO4 buffer solutions and in the presence of urease catalyst, urea can be decomposed to form NH4 (+). The NH4 (+) reacted with sodium tetraphenyl boron (NaTPB) to form the association particles that exhibited a resonance scattering (RS) peak at 474 nm. When the urea concentration increased, NH4 (+) increased, and RS intensity at 474 nm enhanced linearly. Under the chosen conditions, the increased RS intensity (Delta I (474 nm)) had a linear response to the urea concentration in the range of 0.125-15 mu M, with a detection limit of 0.058 mu M urea, and a regression equation of Delta I (474 nm) = 31.6C + 2.1, a correlation coefficient of 0.9986. This catalytic RS method was applied for the detection of urea in human serum sample, with good selectivity and sensitivity, and the results were consistent with the reference method.