Effects of adenosine infusion into renal interstitium on renal hemodynamics.
Effects of adenosine infusion into renal interstitium on renal hemodynamics.
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腺苷输注至肾间质对肾血流动力学的影响。
DOI：
10.1152/ajprenal.1987.252.4.f678
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Knox,FG
中科院分区：
文献类型：
--
作者：
Pawlowska,D;Granger,JP;Knox,FG
本研究旨在探讨外源性腺苷对大鼠肾间质血流动力学的影响。腺苷或其类似物通过长期植入的胶囊输注到肾间质。腺苷输注（0.5 mumol + 0.1 mumol/min）使肾小球滤过率（GFR）从0.81 +/- 0.06（平均+/- SE）降至0.37 +/- 0.06 ml/min，而对肾血流量（RBF）没有影响。代谢稳定的类似物2-氯腺苷（2- clado）（剂量为10 nmol + 2 nmol/min）使GFR从0.73 +/- 0.07降低到0.21 +/- 0.06 ml/min。间质性输注茶碱（一种腺苷受体拮抗剂）完全消除了腺苷和2-ClAdo对GFR的影响。用放射性标记的5'-（n -乙基）-羧氨基腺苷（NECA）（一种代谢稳定的腺苷激动剂）测定输注到肾间质时腺苷的分布。持续输注后，[3H]NECA分布于整个肾脏。NECA降低GFR的作用与腺苷和2-ClAdo相似。我们得出结论，在稳态条件下，肾间质中腺苷水平的增加可显著降低GFR，而不影响RBF。腺苷激动剂在输注肾间质时的显著作用和茶碱对这些作用的完全阻断表明腺苷的细胞外作用。
This study was designed to investigate the hemodynamic effects of exogenous adenosine in the interstitium of the rat kidney. Adenosine or its analogues were infused into the renal interstitium by means of chronically implanted capsules. Infusion of adenosine (bolus 0.5 mumol plus 0.1 mumol/min) decreased glomerular filtration rate (GFR) from 0.81 +/- 0.06 (mean +/- SE) to 0.37 +/- 0.06 ml/min while having no effect on renal blood flow (RBF). The metabolically stable analogue, 2-chloradenosine (2-ClAdo), (bolus 10 nmol plus 2 nmol/min) decreased GFR from 0.73 +/- 0.07 to 0.21 +/- 0.06 ml/min. Interstitial infusion of theophylline, an adenosine receptor antagonist, completely abolished the effects of adenosine and 2-ClAdo on GFR. The distribution of adenosine, when infused into the renal interstitium, was determined using radiolabeled 5'-(N-ethyl)-carboxamidoadenosine (NECA), a metabolically stable adenosine agonist. After continuous infusion, [3H]NECA was distributed throughout the kidney. The effects of NECA to reduce GFR were similar to those of adenosine and 2-ClAdo. We conclude that increased levels of adenosine in the renal interstitium markedly decrease GFR without affecting RBF in steady-state conditions. The marked effects of adenosine agonists during their infusion into the renal interstitium and the complete blockade of these effects by theophylline suggest an extracellular action of adenosine.