Insulin-like growth factor-II gene expression in Wilms' tumour and embryonic tissues
Insulin-like growth factor-II gene expression in Wilms' tumour and embryonic tissues
复制标题
肾母细胞瘤和胚胎组织中胰岛素样生长因子-II 基因的表达
作者：
James Scott;J. Cowell;M. Robertson;L. Priestley;R. Wadey;B. Hopkins;J. Pritchard;G. Bell;L. Rall;C. Graham;T. Knott
肾母细胞瘤是一种遗传性和自发性的胚胎性肿瘤。这两种类型都显示出11号染色体短臂的重排。患有罕见的遗传性无虹膜、泌尿生殖系统异常和智力低下三联症的儿童胚系携带的11号染色体短臂(11p13带)缺失，这些儿童患Wilms肿瘤的风险增加1，2。患有Beckwith-Wiedemann综合征的新生儿，可能存在11p13-11p15区域的重复，也同样易患3。在自发形式的肿瘤中，肿瘤细胞中HP14条带的缺失已被报道，但在正常细胞中不是，4，11P区域隐性突变的纯合发展与Wilms‘s肿瘤的病因学有关5-8。鉴于这些染色体重排，由于Wilms’s肿瘤与肾脏发育的早期阶段历史上无法区分9，我们现在检查了Wilms‘s肿瘤和人类胚胎组织中定位于11p的基因的表达。在12例散发性肿瘤中，定位于11p15区域的胰岛素样生长因子-II(IGF-II)基因的表达水平明显高于成人组织，但与肾脏、肝脏、肾上腺和横纹肌等胎儿组织的表达水平相当。这可能反映了肿瘤分化的阶段，但也可能有助于恶性过程，因为IGF-II是一种胚胎有丝分裂原10-13。
Wilms' tumour (nephroblastoma) is an embryonal neoplasm occurring in hereditary and spontaneous forms. Both types show rearrangements of the short arm of chromosome 11. The germ line of children with the rare inherited triad of aniridia, genitourinary abnormality and mental retardation carry a chromosome 11 that has a deletion in its short arm (band 11p13) and these children are at increased risk of developing Wilms' tumour1,2. Neonates with the Beckwith–Wiedemann syndrome, in which there may be duplication of the 11p13–11p15 region, are similarly predisposed3. In the spontaneous form of the tumour a deletion of the Hp14 band in tumour cells, but not in normal cells, has been reported4, and the development of homozygosity for recessive mutations in the 11p region is implicated in the aetiology of Wilms' tumour5–8. In view of these chromosomal rearrangements and because Wilms' tumour is historically indistinguishable from the early stages of kidney development9, we have now examined the expression of genes localized to 11p in Wilms' tumour and human embryonic tissue. In 12 sporadic tumours examined, the expression of the gene coding for insulin-like growth factor-II (IGF-II), localized to the 11p15 region, was markedly increased relative to adult tissues, but was comparable to the level of expression in several fetal tissues including kidney, liver, adrenals and striated muscle. This may reflect the stage of tumour differentiation, but could also contribute to the malignant process, as IGF-II is an embryonal mitogen10–13.
11 号染色体短臂间质性缺失仅限于无虹膜患者的肾母细胞瘤细胞。
影响因子：
11.2
作者：
Kaneko,Y;Egues,MC;Rowley,JD
通讯作者：
Rowley,JD