Diversity and similarity among recognition sequences of Dof transcription factors.
Diversity and similarity among recognition sequences of Dof transcription factors.
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DOI：
10.1046/j.1365-313x.1999.00363.x
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
S. Yanagisawa;R. Schmidt
中科院分区：
文献类型：
--
作者：
S. Yanagisawa;R. Schmidt
Dof蛋白是一类具有独特DNA结合结构域的转录因子家族。近年来发现Dof蛋白与多种植物特异性基因的启动子相关，提示Dof蛋白在植物中具有多种功能。利用随机合成的DNA进行结合位点选择实验，系统分析了4个玉米Dof蛋白的识别序列。所有选择的寡核苷酸含有AAAG序列，表明该序列是Dof蛋白的识别核心。事实上，该序列中的单个突变消除了所有四种Dof蛋白的结合。此外，还使用含有固定AAAG和随机侧翼序列的寡核苷酸分析了每个Dof蛋白对核心基序侧翼序列的偏好。在最佳结合位点之间观察到相似以及不同的侧翼序列。侧翼序列的变化确实影响Dof蛋白的DNA结合。
Dof proteins are a family of transcription factors that share a unique DNA-binding domain. Dof proteins were found recently in association with diverse promoters of plant-specific genes, suggesting various roles of Dof proteins in plants. Through binding site selection experiments using randomly synthesized DNA, the recognition sequences of four maize Dof proteins were systematically analyzed. All selected oligonucleotides contained an AAAG sequence, suggesting that this sequence is the recognition core of Dof proteins. In fact, a single mutation in this sequence abolished binding of all four Dof proteins. Furthermore, the preference of each Dof protein for the sequence flanking the core motif was also analyzed using oligonucleotides containing a fixed AAAG and random flanking sequences. Similar, as well as distinct, flanking sequences were observed among the optimal binding sites. Changes in the flanking sequences did affect DNA-binding of Dof proteins.