Periodic subcellular structures undergo long-range synchronized reorganization during C. elegans epidermal development
Periodic subcellular structures undergo long-range synchronized reorganization during C. elegans epidermal development
复制标题
DOI：
10.1242/jcs.246793
复制
发表时间：
2020-10
影响因子：
4
通讯作者：
Chunxia Wang;Yuyan Yang;Rong Fu;Yi Zhu;Huimin Zhang
中科院分区：
文献类型：
--
作者：
Chunxia Wang;Yuyan Yang;Rong Fu;Yi Zhu;Huimin Zhang
细胞和组织尺度上的周期性图案形成是一个重要的过程，并已被广泛研究。然而，在亚细胞水平上的周期性图案形成仍然知之甚少。梭线虫表皮显示高度有序的平行条纹图案作为其亚细胞结构的一部分，使其成为研究细胞内周期性图案的形成和重组的理想模型。在这里，我们表明，在C。线虫表皮依赖于肌动蛋白和血影蛋白，并且需要顶端膜附着结构来维持。周期性的亚细胞结构不通过不断地制造新的条纹来适应细胞生长。相反，它们通过一轮均匀的复制来增加条纹的数量，这与表皮长度的增加或发育周期无关。这种亚细胞结构的长距离同步重组是通过细胞外胶原与表皮细胞生长产生的伸展力建立的物理联系实现的。我们的研究揭示了均匀间隔和相互连接的亚细胞结构在细胞生长和组织发育过程中保持其完整性和均匀分布的新策略。下一篇：使用C。以线虫表皮为模型，我们发现周期性组织的亚细胞结构进行同步复制，以增加其数量并适应表皮细胞的生长。
ABSTRACT Periodic pattern formation on the cellular and tissue scale is an important process and has been extensively studied. However, periodic pattern formation at the subcellular level still remains poorly understood. The C. elegans epidermis displays a highly ordered parallel stripe pattern as part of its subcellular structure, making it an ideal model to study the formation and reorganization of periodic patterns within cells. Here, we show that the initial formation of periodic striped patterns in the C. elegans epidermis is dependent on actin and spectrin, and requires the apical membrane attachment structures for maintenance. The periodic subcellular structures do not accommodate cell growth by continuously making new stripes. Instead, they increase the number of stripes by going through one round of uniform duplication, which is independent of the increasing epidermal length or the developmental cycles. This long-range synchronized reorganization of subcellular structures is achieved by physical links established by extracellular collagens together with extension forces generated from epidermal cell growth. Our studies uncover a novel strategy employed by evenly spaced and interlinked subcellular structures to maintain their integrity and equidistribution during cell growth and tissue development. Highlighted Article: By using C. elegans epidermis as a model, we find that periodically organized subcellular structures perform synchronized duplication to increase their numbers and accommodate epidermal cell growth.