Layer formation of 2,5-dihydroxybenzoic acid crystals via mist deposition for mass spectrometry imaging of biological samples
Layer formation of 2,5-dihydroxybenzoic acid crystals via mist deposition for mass spectrometry imaging of biological samples
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DOI：
10.35848/1882-0786/abd6a1
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发表时间：
2021-02
影响因子：
2.3
通讯作者：
Yuji Nakabayashi;H. Sakai;Akio Miyazato;Chiko Shijimaya;Ryoichi Suzuki;S. Yamada
中科院分区：
文献类型：
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作者：
Yuji Nakabayashi;H. Sakai;Akio Miyazato;Chiko Shijimaya;Ryoichi Suzuki;S. Yamada
通过喷雾沉积形成二羟基苯甲酸（DHB）晶体层。在200 °C的喷嘴温度下形成显示测量为几百微米的晶须的晶体层。DHB晶体层在紫外波长区域表现出强吸收，并且拉曼光谱证实了它们的振动性质。通过基质辅助激光解吸/电离飞行时间质谱成像使用DHB晶体层作为质量电离辅助剂分析脂质组分。胆固醇、二酰甘油和甘油三酯被检测为钠加合物。通过防止与分析物的非均相共结晶，质量图像比使用常规气溶胶喷雾方法时更清晰。
Dihydroxybenzoic acid (DHB) crystal layers were formed via mist deposition. Crystal layers exhibiting whiskers measuring a few hundred micrometers were formed at a nozzle temperature of 200 °C. DHB crystal layers exhibited strong absorbance in the ultra-violet wavelength regions, and Raman spectroscopy confirmed their vibrational property. The lipid component was analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry imaging using a DHB crystal layer as the mass ionization assist agent. Cholesterols, diacylglycerides, and triglycerides were detected as sodium adducts. By preventing heterogeneous co-crystallization with analytes, mass images were clearer than when using the conventional aerosol spray method.