Effect on the Expression of MMP-2, MT-MMP in Laryngeal Carcinoma Hep-2 Cell Line by Antisense Glucose Transporter-1
Effect on the Expression of MMP-2, MT-MMP in Laryngeal Carcinoma Hep-2 Cell Line by Antisense Glucose Transporter-1
复制标题
反义葡萄糖转运蛋白1对喉癌Hep-2细胞系MMP-2、MT-MMP表达的影响
DOI：
10.1016/j.arcmed.2012.07.003
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发表时间：
2012-07-01
影响因子：
7.7
通讯作者：
Fan, Jun
中科院分区：
文献类型：
--
作者：
Xu, Ying-Ying;Bao, Yang-Yang;Fan, Jun
背景和目标。葡萄糖转运蛋白-1（Glut-1）与恶性肿瘤的生物学行为密切相关。然而，没有证据表明Glut-1的过表达在机制上导致癌细胞的侵袭或转移。我们假设Glut-1调节膜型1-MMP（MT 1-MMP）和基质金属蛋白酶-2（MMP-2）的表达。采用RT-PCR分析Glut-1、MMP-2、beta-actin和MT 1-MMP的表达。Western blot检测Glut-1蛋白、MMP-2、MT 1-MMP的表达。在mRNA和蛋白水平上，Glut-1和MMP-2在Hep-2喉癌细胞系中共表达。转染后，Glut-1反义寡核苷酸（AS-ODN）可降低MMP-2和Glut-1 mRNA和蛋白的表达。Glut-1 AS-ODN可降低MT 1-MMP mRNA的表达。Glut-1和MMP-2在Hep-2喉癌细胞中共表达，Glut-1可能调节MMP-2和MT 1-MMP的表达。(C)2012年IMSS。爱思唯尔公司出版
Background and Aims. Glucose transporter protein-1 (Glut-1) is correlated with biological behaviors of malignant tumors. However, there was no evidence that overexpression of Glut-1 mechanistically lead to invasion or metastasis of cancer cells. We hypothesized that Glut-1 regulates the expression of membrane type 1-MMP (MT1-MMP) and matrix metalloproteinase-2 (MMP-2).Methods. Analysis of the expression of Glut-1, MMP-2, beta-actin, and MT1-MMP was performed using RT-PCR. Expression of Glut-1 protein, MMP-2, and MT1-MMP was detected by Western blotting.Results. At mRNA and protein levels, Glut-1 and MMP-2 were co-expressed in the Hep-2 laryngeal carcinoma cell line. After transfection, Glut-1 antisense oligodeoxynucleotide (AS-ODN) decreased the expression of MMP-2 mRNA and protein as well as Glut-1 mRNA and protein. Glut-1 AS-ODN also decreased the expression of MT1-MMP mRNA.Conclusions. Co-expression of Glut-1 and MMP-2 in Hep-2 laryngeal carcinoma cells and Glut-1 may regulate MMP-2 and MT1-MMP expression. (C) 2012 IMSS. Published by Elsevier Inc.