Two Fungicides Alter Reproduction of the Small Brown Planthopper Laodelphax striatellus by Influencing Gene and Protein Expression.
Two Fungicides Alter Reproduction of the Small Brown Planthopper Laodelphax striatellus by Influencing Gene and Protein Expression.
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DOI：
10.1021/acs.jproteome.7b00612
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发表时间：
2018-02
影响因子：
4.4
通讯作者：
You Wu;Jun-nan Ding;Bing Xu;L. You;L. Ge;Guoqing Yang;Fang Liu;D. Stanley;Q. Song;Jin‐cai Wu
中科院分区：
文献类型：
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作者：
You Wu;Jun-nan Ding;Bing Xu;L. You;L. Ge;Guoqing Yang;Fang Liu;D. Stanley;Q. Song;Jin‐cai Wu
除预期作用外，由于几乎持续使用和害虫进化，杀菌剂可能导致害虫暴发。小褐飞虱（ladelphax striatellus）最近在中国许多省份暴发，造成了毁灭性的水稻损失。由于暴露于杀菌剂井冈霉素（JGM）通过影响脂肪酸合成酶而增加褐飞虱（Nilaparvata lugens）的繁殖，我们提出了JGM和多菌灵（CBM）通过影响脂质代谢其他方面的酶来影响褐飞虱繁殖的假设。杀菌剂CBM刺激了SBPH的繁殖（产卵量增加78%），而另一种杀菌剂JGM则导致产卵量减少（减少47.3%）。这些反向效应是由jgm处理雌性小鼠中l-3-羟基酰基辅酶A脱氢酶（LCHAD）表达下调和cbm处理雌性小鼠中羟基类固醇脱氢酶样蛋白2样（HSD）表达上调介导的。RNAi分别敲低LCHAD和HSD导致产蛋量减少（dsLCHAD减少52%，dsHSD减少73%）。dsLCHAD、dsHSD和JGM处理也导致实验性SBPH患者卵巢发育严重降低，瓣膜变短变薄，卵巢中缺乏卵细胞。经cbm处理的雌虫瓣膜增大，卵巢内有香蕉形卵。这些数据有力地支持了我们的假设。
Aside from their intended actions, fungicides can drive pest insect outbreaks due to virtually continuous use and pest evolution. Small brown planthopper (SBPH), Laodelphax striatellus, outbreaks occurred recently in many provinces in China, with devastating rice losses. Because exposure to the fungicide jinggangmycin (JGM) increased reproduction of the brown plant hopper, Nilaparvata lugens, via its influence on fatty acid synthase, we posed the hypothesis that JGM and carbendazim (CBM) influence SBPH reproduction via their influence on enzymes involved in other aspects of lipid metabolism. Exposure to the fungicide CBM stimulated SBPH reproduction (egg-laying up by 78%) and to another fungicide, JGM, led to decreased egg-laying (down by 47.3%). These inverse effects are mediated by down-regulated expression of l-3-hydroxyacyl-coenzyme A dehydrogenase (LCHAD) in JGM-treated females and up-regulated expression of hydroxysteroid dehydrogenase-like protein 2-like (HSD) in CBM-treated females. RNAi knockdown of, separately, LCHAD and HSD led to reduced egg-laying (down by 52% for dsLCHAD and by 73% for dsHSD). dsLCHAD, dsHSD, and JGM treatments also led to severely reduced ovarian development in experimental SBPH, with shorted and thinned valvula and lack of egg cells in ovaries. Valvula of CBM-treated females enlarged, with banana-shaped eggs in ovaries. These data strongly support our hypothesis.