Structure and function of microbial community involved in a novel full-scale prefix oxic coking wastewater treatment O/H/O system
Structure and function of microbial community involved in a novel full-scale prefix oxic coking wastewater treatment O/H/O system
复制标题
新型全尺寸前缀含氧焦化废水处理O/H/O系统中微生物群落的结构和功能
DOI：
10.1016/j.watres.2019.114963
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发表时间：
2019
期刊：
影响因子：
12.8
通讯作者：
Wei Chaohai
中科院分区：
文献类型：
--
作者：
Zhu Shuang;Wu Haizhen;Wu Chaofei;Qiu Guanglei;Feng Chunhua;Wei Chaohai
新型全线前置含氧焦化废水（CWW）生物处理O/H/O系统已稳定运行六年，出水水质达到国家排放标准。与传统的CWW生物处理工艺（通常在工艺开始时设有厌氧单元）相比，O/H/O系统在去除COD、去除总氮和降低能耗方面具有明显的优势。阐明O/H/O系统不同生物反应器中涉及的微生物群落的结构和功能是非常有必要的。高通量 MiSeq 测序用于检查该系统中的 16S rRNA 基因。结果表明，三个连续生物反应器的活性污泥样品中微生物组成存在差异：O1活性污泥中以β-Proteobacteria相关序列为主，相对丰度为56.44%，硫杆菌占7.53%；H和O2活性污泥中Rhodoplanes相关序列占主导地位，相对丰度为7.53%。 丰度分别为8.86%和8.92%。通过标准方法分析 CWW 的物理化学特性，并定期监测操作参数以检查其对微生物群落的影响。利用未观察状态重建群落系统发育生物信息学软件包（PICRUSt）对微生物群落功能进行预测，发现红平面菌属、溶杆菌属和白杆菌属三个优势属丰富了外源物质的生物降解和代谢途径。 CWW处理的生物处理过程涉及多样且独特的微生物群落，这表明水的特性和操作参数决定了微生物群落的组成。这些结果显着扩展了我们对微生物生物多样性和种群动态的了解，并揭示了生物处理过程中细菌群落与环境变量之间的关系。此外，在本研究中，我们提出了CWW处理的综合生物降解模型，并将其定义为O/H/O系统。
A novel full-scale prefix oxic coking wastewater (CWW) biological treatment O/H/O system had been operated steadily six years with the effluent quality meeting national discharge standard. Comparing to the traditional CWW biological treatment process, which usually have an anaerobic unit at the start of the process, here the O/H/O system has obvious advantages in COD removal, total nitrogen removal and reduced energy consumption. It is very necessary to illustrate the structure and function of the microbial community involved in different bioreactors of the O/H/O system. High-throughput MiSeq sequencing was used to examine the 16S rRNA genes in this system. Results revealed a contrasting microbial composition among the activated sludge samples of three sequential bioreactors: theβ-Proteobacteriarelated sequences dominated in the O1activated sludge with the relative abundance of 56.44% while 7.53% of the sequences were assigned to Thiobacillus;Rhodoplanesrelated sequences dominated in the bioreactor H and O2activated sludge with relative abundance of 8.86% and 8.92%, respectively. The physico-chemical characteristics of CWW were analyzed by standard methods and the operational parameters were routinely monitored to examine their effects on the microbial communities. The bioinformatics software package of phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) was used to predict the microbial community functional profiling and found three dominant genera ofRhodoplanes,LysobacterandLeucobacterenriched the xenobiotics biodegradation and metabolism pathway. The diverse and distinct microbial community involved in biological treatment processes of CWW treatment indicating that water characteristics and operational parameters determined the microbial community composition. These results significantly expanded our knowledge of the biodiversity and population dynamics of microorganisms and discerned the relationships between bacterial communities and environmental variables in the biological treatment processes. Moreover, in this study, we proposed a comprehensive biodegradation model of CWW treatment and defined as O/H/O system.