Regulating size of silver nanoparticles on calcium carbonate via ultrasonic spray for effective antibacterial efficacy and sustained release
Regulating size of silver nanoparticles on calcium carbonate via ultrasonic spray for effective antibacterial efficacy and sustained release
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DOI：
10.1016/j.msec.2021.112083
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发表时间：
2021-04-06
影响因子：
7.9
通讯作者：
Aizawa, Mamoru
中科院分区：
文献类型：
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作者：
Ueda, Mayu;Yokota, Tomohiro;Aizawa, Mamoru
碳酸钙因其良好的生物相容性、生物活性和生物可吸收性而被用作骨填充材料，但与碳酸钙相关的感染性并发症的流行给骨缺损的治疗带来了持续的挑战。因此，这极大地需要赋予碳酸钙抗菌性能。本研究以碳酸钙粉体为载体，制备了载银纳米粒子（Ag-CaCO 3）抗菌无机填料。使用超声喷雾热解（USSP）路线来生产具有1、5和10摩尔%银的Ag-CaCO 3来实现该目的。通过调节银的浓度可以调节碳酸钙微球上银纳米颗粒的大小，以促进Ag+离子的有效释放。这在Ag-CaCO 3（1）中得到了证实，其中最低银含量为1 mol%时，在28天内实现了最高的Ag+离子释放。这反过来又对金黄色葡萄球菌和大肠杆菌产生了有效的抗菌效率。此外，CaCO 3（1）也可以支持成骨细胞样细胞（MG-63），细胞活力为80%。总的来说，这项工作扩展了使用USSP生产具有持续抗菌性能的无机填料材料的能力，使抗菌产品的开发更近了一步。
Calcium carbonate is used as bone-filling material due to its good biocompatibility, bioactivity, and bioabsorbability, but the prevalence of infectious complications associated with calcium carbonate has created a persisting challenge in the treatment of bone defect. Therefore, this greatly necessitate the need to endow calcium carbonate with antibacterial properties. In this study, calcium carbonate powders loaded with silver nanoparticles (Ag-CaCO3) were prepared in attempt to serve as a novel antibacterial inorganic filler material. This objective was achieved using ultrasonic spray-pyrolysis (USSP) route to produce Ag-CaCO3 with 1, 5 and 10 mol% silver. The size of silver nanoparticles on CaCO3 microspheres could be regulated by adjusting silver concentration to facilitate effective release of Ag+ ions. This was demonstrated in Ag-CaCO3 (1), where the lowest silver content at 1 mol% achieved the highest Ag+ ions release over 28 days. This in turn gave rise to effective antibacterial efficiency against Staphylococcus aureus and Escherichia coli. Furthermore, CaCO3 (1) could also support osteoblast-like cells (MG-63) at a cell viability of 80%. Overall, this work extends the capabilities in employing USSP to produce inorganic filler materials with sustained antibacterial properties, bringing one step closer to the development of antibacterial products.