MICROMOTION OF MAMMALIAN-CELLS MEASURED ELECTRICALLY
MICROMOTION OF MAMMALIAN-CELLS MEASURED ELECTRICALLY
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DOI：
10.1073/pnas.88.17.7896
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发表时间：
1991-09-01
影响因子：
11.1
通讯作者：
KEESE, CR
中科院分区：
文献类型：
--
作者：
GIAEVER, I;KEESE, CR
运动性是哺乳动物细胞的基本性质，通常在组织培养中通过延时显微镜观察到，其中分辨率受光的波长限制。本文探讨了一个强大的电子技术，细胞运动的定量测量在纳米水平。在这种方法中，细胞在携带弱交流电流的小蒸发金电极上培养。当细胞在这些电极上附着和扩散时，观察到电极的测量电阻抗的大的变化。当作为时间的函数跟踪阻抗时，观察到作为细胞运动的直接测量的波动。令人惊讶的是，即使细胞层变得融合，这些波动也会继续。通过将测量的阻抗与理论模型进行比较，可以清楚地看出，在这些情况下，可以从测量结果推断出细胞层的平均运动为1 nm。我们把细胞运动的这一方面称为微运动。
Motility is a fundamental property of mammalian cells that normally is observed in tissue culture by time lapse microscopy where resolution is limited by the wavelength of light. This paper examines a powerful electrical technique by which cell motion is quantitatively measured at the nanometer level. In this method, the cells are cultured on small evaporated gold electrodes carrying weak ac currents. A large change in the measured electrical impedance of the electrodes is observed when cells attach and spread on these electrodes. When the impedance is tracked as a function of time, fluctuations are observed that are a direct measure of cell motion. Surprisingly, these fluctuations continue even when the cell layer becomes confluent. By comparing the measured impedance with a theoretical model, it is clear that under these circumstances the average motions of the cell layer of 1 nm can be inferred from the measurements. We refer to this aspect of cell motility as micromotion.