Biotoxin Tropolone Contamination Associated with Nationwide Occurrence of Pathogen Burkholderia plantarii in Agricultural Environments in China
Biotoxin Tropolone Contamination Associated with Nationwide Occurrence of Pathogen Burkholderia plantarii in Agricultural Environments in China
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生物毒素托酚酮污染与中国农业环境中植物伯克霍尔德杆菌在全国范围内的发生有关
DOI：
10.1021/acs.est.7b05915
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发表时间：
2018-05-01
影响因子：
11.4
通讯作者：
Wang, Mengcen
中科院分区：
文献类型：
--
作者：
Liu, Xiaoyu;Fan, Xiaoyan;Wang, Mengcen
环庚三烯酚酮是由植物伯克霍尔德氏菌产生的一种生物毒素，对多种生物具有细胞毒性。然而，由于缺乏定量和定性的方法，无论是B。对农业环境中的车前草发生和环酚酮污染仍然知之甚少。本研究为B的检测提供了一种灵敏可靠的方法。植物，人工和环境基质中的车前草，通过tropolone为目标的气相色谱-三重四极杆串联质谱分析。B的检测限。车前草和托酚酮分别为10个菌落形成单位（CFU）/mL和0.017 μ g/kg。在一系列模拟试验中，我们发现B. 10至108 CFU/mL的plantarii以细胞群体依赖性方式产生0.006至107.8 mg/kg的托酚酮，而与栖息地无关。相关分析阐明了B的可靠反映。不同浓度的托酚酮对车前草密度影响的相关系数为0.9201 ~ 0.9756（p < 0.01）。通过在中国进行的一项全国性试点研究，在水稻土和水稻籽粒中观察到0.014-0.157 mg/kg的酚酮污染，随后的冗余分析表明，土壤有机质是一个主导的环境因子，与酚酮污染呈正相关。在这种情况下，我们的研究结果意味着，潜在的生态和饮食风险所造成的长期暴露于痕量的环庚三烯酚酮污染的关注。
Tropolone, a biotoxin produced by the agricultural pathogen Burkholderia plantarii, exerts cytotoxicity toward a wide array of biota. However, due to the lack of quantitative and qualitative approach, both B. plantarii occurrence and tropolone contamination in agricultural environments remain poorly understood. Here, we presented a sensitive and reliable method for detection of B. plantarii in artificial, plant, and environmental matrices by tropolone-targeted gas chromatography-triple-quadrupole tandem mass spectrometry analysis. Limits of detection for B. plantarii and tropolone were 10 colony-forming units (CFU)/mL and 0.017 mu g/kg, respectively. In a series of simulation trials, we found that B. plantarii from 10 to 108 CFU/mL produced tropolone between 0.006 and 107.8 mg/kg in a cell-population-dependent manner, regardless of habitat. Correlation analysis clarified a reliable reflection of B. plantarii density by tropolone level with R-2 values from 0.9201 to 0.9756 (p < 0.01). Through a nationwide pilot study conducted in China, tropolone contamination was observed at 0.014-0.157 mg/kg in paddy soil and rice grains, and subsequent redundancy analysis revealed soil organic matter to be a dominant environmental factor, having a positive correlation with tropolone contamination. In this context, our results imply that potential ecological and dietary risks posed by long-term exposure to trace levels of tropolone contamination are of concern.