Up-regulation of Na+K+-ATPase α3-isoform and down-regulation of the α1-isoform in human colorectal cancer
Up-regulation of Na+K+-ATPase α3-isoform and down-regulation of the α1-isoform in human colorectal cancer
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DOI：
10.1016/s0014-5793(04)00292-3
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发表时间：
2004-04-09
期刊：
影响因子：
3.5
通讯作者：
Takeguchi, N
中科院分区：
文献类型：
--
作者：
Sakai, H;Suzuki, T;Takeguchi, N
We investigated expression levels of Na+,K+-ATPase alpha-isoforms and their ATPase activities in human colorectal cancer tissue and the accompanying normal mucosa. A decrease in expression of the alpha1-isoform protein was observed in all sampled cancer tissues compared with the normal mucosae. The level of ouabain (5 muM)-sensitive Na+,K+-ATPase activity in carcinomas was 81 +/- 5% that of in the normal mucosae. The mRNA expression of alpha2- and alpha4-isoforms was decreased in almost all the carcinoma samples. Interestingly, the expression level of the alpha3-isoform protein in the cancer tissue was higher than that of the normal mucosa. These results indicate that a decrease in the alpha1-isoform expression and an increase in the alpha3-isoform expression may be associated with colorectal cancer. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
