Osteopontin is expressed by adult human osteoarthritic chondrocytes: protein and mRNA analysis of normal and osteoarthritic cartilage
Osteopontin is expressed by adult human osteoarthritic chondrocytes: protein and mRNA analysis of normal and osteoarthritic cartilage
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DOI：
10.1016/s0945-053x(00)00068-8
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发表时间：
2000-07-01
期刊：
影响因子：
6.9
通讯作者：
Swoboda, B
中科院分区：
文献类型：
--
作者：
Pullig, O;Weseloh, G;Swoboda, B
骨桥蛋白是一种具有细胞结合和基质结合特性的硫酸化磷蛋白，在多种组织中表达。在胚胎生长板，骨桥蛋白的表达被发现在骨形成细胞和肥大的软骨细胞。在这项研究中，骨桥蛋白的表达进行了分析，在正常和骨关节炎的人膝关节软骨。免疫组化，使用单克隆抗骨桥蛋白抗体是阴性的正常软骨。这些结果在Western印迹实验中得到证实，使用部分纯化的正常膝关节软骨提取物。成人健康软骨的软骨细胞未见骨桥蛋白基因表达，而软骨下骨板的成骨细胞有骨桥蛋白mRNA表达。骨关节炎患者软骨中骨桥蛋白可通过免疫组化、Western印迹分析、原位杂交和北方印迹分析检测到。定性分析表明，骨桥蛋白的蛋白沉积和mRNA的表达增加与骨关节炎病变的严重程度和软骨基质的崩解。骨桥蛋白在软骨中的表达仅限于上深区的软骨细胞，显示细胞和领土沉积。最强的骨桥蛋白检测被发现在深区软骨细胞和集群的增殖软骨细胞的样本与严重的骨关节炎病变。这些数据显示骨桥蛋白在成人骨关节炎软骨细胞中的表达，表明软骨细胞分化和骨关节炎软骨中分化标志物的表达类似于骨骺生长板软骨细胞。(C)2000 Elsevier Science B.V./国际矩阵生物学学会。All rights reserved.
Osteopontin, a sulfated phosphoprotein with cell binding and matrix binding properties, is expressed in a variety of tissues. In the embryonic growth plate, osteopontin expression was found in bone-forming cells and in hypertrophic chondrocytes. In this study, the expression of osteopontin was analyzed in normal and osteoarthritic human knee cartilage. Immunohistochemistry, using a monoclonal anti-osteopontin antibody was negative on normal cartilage. These results were confirmed in Western blot experiments, using partially purified extracts of normal knee cartilage. No osteopontin gene expression was observed in chondrocytes of adult healthy cartilage, however, in the subchondral bone plate, expression of osteopontin mRNA was detected in the osteoblasts. In cartilage from patients with osteoarthritis, osteopontin could be detected by immunohistochemistry, Western blot analysis, in situ hybridization, and Northern blot analysis. A qualitative analysis indicated that osteopontin protein deposition and mRNA expression increase with the severity of the osteoarthritic lesions and the disintegration of the cartilaginous matrix. Osteopontin expression in the cartilage was limited to the chondrocytes of the upper deep zone, showing cellular and territorial deposition. The strongest osteopontin detection was found in deep zone chondrocytes and in clusters of proliferating chondrocytes from samples with severe osteoarthritic lesions. These data show the expression of osteopontin in adult human osteoarthritic chondrocytes, suggesting that chondrocyte differentiation and the expression of differentiation markers in osteoarthritic cartilage resembles that of epiphyseal growth plate chondrocytes. (C) 2000 Elsevier Science B.V./International Society of Matrix Biology. All rights reserved.