Glycosaminoglycans in human retinoblastoma cells: heparan sulfate, a modulator of the pigment epithelium-derived factor-receptor interactions.
Glycosaminoglycans in human retinoblastoma cells: heparan sulfate, a modulator of the pigment epithelium-derived factor-receptor interactions.
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DOI：
10.1186/1471-2091-4-1
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发表时间：
2003-02-19
期刊：
影响因子：
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通讯作者：
Becerra, S Patricia
中科院分区：
文献类型：
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作者：
Alberdi, Elena M;Weldon, John E;Becerra, S Patricia
背景：色素上皮源性因子(PEDF)与视网膜母细胞瘤细胞表面受体和糖胺聚糖具有亲和力。结果：125I-PEDF与人视网膜母细胞瘤Y-79细胞条件培养液中的蛋白酶抗性成分形成了复合体。用分光光度法和PEDF亲和层析法检测到Y-79条件培养液中与PEDF具有结合亲和力的肝素和类硫酸肝素糖胺聚糖。Y-79条件培养液可显著增强125I-PEDF与Y-79细胞表面受体的结合。然而，肝素酶和氯酸盐处理去除Y-79细胞培养上清液中的硫酸氨基葡聚糖，降低了125I-PEDF与细胞表面受体的结合程度。结论：这些数据表明，视网膜母细胞瘤细胞分泌与PEDF具有结合亲和力的肝素/硫酸肝素，这可能在有效的细胞-表面受体结合中起重要作用。
BACKGROUND: Pigment epithelium-derived factor (PEDF) has binding affinity for cell-surface receptors in retinoblastoma cells and for glycosaminoglycans. We investigated the effects of glycosaminoglycans on PEDF-receptor interactions.RESULTS: 125I-PEDF formed complexes with protease-resistant components of medium conditioned by human retinoblastoma Y-79 cells. Using specific glycosaminoglycan degrading enzymes in spectrophotometric assays and PEDF-affinity chromatography, we detected heparin and heparan sulfate-like glycosaminoglycans in the Y-79 conditioned media, which had binding affinity for PEDF. The Y-79 conditioned media significantly enhanced the binding of 125I-PEDF to Y-79 cell-surface receptors. However, enzymatic and chemical depletion of sulfated glycosaminoglycans from the Y-79 cell cultures by heparitinase and chlorate treatments decreased the degree of 125I-PEDF binding to cell-surface receptors.CONCLUSIONS: These data indicate that retinoblastoma cells secrete heparin/heparan sulfate with binding affinity for PEDF, which may be important in efficient cell-surface receptor binding.