Expression profiles of proton-sensing G-protein coupled receptors in common skin tumors.
Expression profiles of proton-sensing G-protein coupled receptors in common skin tumors.
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DOI：
10.1038/s41598-020-71700-9
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发表时间：
2020-09-18
影响因子：
4.6
通讯作者：
Schreml S
中科院分区：
文献类型：
--
作者：
Klatt W;Wallner S;Brochhausen C;Stolwijk JA;Schreml S
质子感应GPCR（pH-GPCR）GPR 4（GPR 19）、TDAG 8（GPR 65，T细胞死亡相关基因8）、OGR 1（GPR 68，卵巢癌GPCR 1）和G2 A（GPR 132，G2积累蛋白）参与细胞外pH（pHe）的感应和转导变化。细胞外酸化是实体癌的主要标志。pH-GPCR功能与癌细胞增殖、粘附、迁移和转移以及免疫系统的调节有关。关于pH-GPCR在皮肤癌中的表达水平和作用知之甚少。为了更好地了解pH-GPCR在皮肤癌中的作用，我们检测了GPR 4、TDAG 8、OGR 1和G2 A在4种常见皮肤肿瘤（鳞状细胞癌（SCC）、恶性黑色素瘤（MM）、复合痣细胞痣（NCN）和基底细胞癌（BCC））中的表达谱。我们对从SCC、MM、NCN或BCC患者获得的石蜡包埋组织样本进行免疫组织化学和免疫荧光染色。我们显示了pH-GPCR在四种常见皮肤癌中的表达。在所研究的皮肤癌类型中的不同表达模式表明，不同的pH-GPCR可能在肿瘤进展中具有不同的功能，并可作为新的治疗靶点。
The proton-sensing GPCRs (pH-GPCRs) GPR4 (GPR19), TDAG8 (GPR65, T-cell death associated gene 8), OGR1 (GPR68, ovarian cancer GPCR1), and G2A (GPR132, G2 accumulation protein) are involved in sensing and transducing changes in extracellular pH (pHe). Extracellular acidification is a central hallmark of solid cancer. pH-GPCR function has been associated with cancer cell proliferation, adhesion, migration and metastasis, as well as with modulation of the immune system. Little is known about the expression levels and role of pH-GPCRs in skin cancer. To better understand the functions of pH-GPCRs in skin cancer in vivo, we examined the expression-profiles of GPR4, TDAG8, OGR1 and G2A in four common skin tumors, i.e. squamous cell carcinoma (SCC), malignant melanoma (MM), compound nevus cell nevi (NCN), basal cell carcinoma (BCC). We performed immunohistochemistry and immunofluorescence staining on paraffin-embedded tissue samples acquired from patients suffering from SCC, MM, NCN or BCC. We show the expression of pH-GPCRs in four common skin cancers. Different expression patterns in the investigated skin cancer types indicate that the different pH-GPCRs may have distinct functions in tumor progression and serve as novel therapeutic targets.
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影响因子：
4.9
作者：
de Vallière C;Wang Y;Eloranta JJ;Vidal S;Clay I;Spalinger MR;Tcymbarevich I;Terhalle A;Ludwig MG;Suply T;Fried M;Kullak-Ublick GA;Frey-Wagner I;Scharl M;Seuwen K;Wagner CA;Rogler G
通讯作者：
Rogler G
影响因子：
8
作者：
Damsky WE;Bosenberg M
通讯作者：
Bosenberg M
影响因子：
--
作者：
Li J;Guo B;Wang J;Cheng X;Xu Y;Sang J
通讯作者：
Sang J
影响因子：
3.7
作者：
Jing Z;Xu H;Chen X;Zhong Q;Huang J;Zhang Y;Guo W;Yang Z;Ding S;Chen P;Huang Z
通讯作者：
Huang Z
影响因子：
6.5
作者：
Hattori, Tomoyasu;Obinata, Hideru;Izumi, Takashi
通讯作者：
Izumi, Takashi