Egfr signaling regulates ommatidial rotation and cell motility in the Drosophila eye via MAPK/Pnt signaling and the Ras effector Canoe/AF6
Egfr signaling regulates ommatidial rotation and cell motility in the Drosophila eye via MAPK/Pnt signaling and the Ras effector Canoe/AF6
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DOI：
10.1242/dev.00759
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发表时间：
2003-11-01
期刊：
影响因子：
4.6
通讯作者：
Mlodzik, M
中科院分区：
文献类型：
--
作者：
Gaengel, K;Mlodzik, M
表皮生长因子受体（Egfr）信号转导在进化上是保守的，控制着各种不同的细胞过程。在果蝇中，这些包括增殖，模式化，细胞命运决定，迁移和生存。在这里，我们提供的证据，一个新的作用，Egfr信号在控制小眼旋转平面细胞极性（PCP）建立在果蝇眼睛。虽然PCP建立和感光细胞类型特化的信号通路开始被解开，但对相关的90度旋转过程知之甚少。其中一个已知的旋转特异性突变是轮盘（rlt），其中小眼旋转到一个随机的角度，通常超过90度。在这里，我们表明，rlt是一个旋转特异性等位基因的抑制性Egfr配体Argos和Egfr活性的调制显示缺陷小眼旋转。我们的数据表明，除了Raf/MAPK级联反应，Ras效应器Canoe/AF 6作用于Egfr/Ras下游，并在此发育调节的细胞运动过程中提供了从Egfr到细胞骨架元素的联系。我们提供了进一步的证据参与钙粘蛋白和非肌肉肌球蛋白II作为下游组件控制旋转。特别是，参与钙粘蛋白火烈鸟，PCP基因，下游的Egfr信号提供了第一个链接之间的PCP建立和Egfr途径。
Epidermal Growth Factor-receptor (Egfr) signaling is evolutionarily conserved and controls a variety of different cellular processes. In Drosophila these include proliferation, patterning, cell-fate determination, migration and survival. Here we provide evidence for a new role of Egfr signaling in controlling ommatidial rotation during planar cell polarity (PCP) establishment in the Drosophila eye. Although the signaling pathways involved in PCP establishment and photoreceptor cell-type specification are beginning to be unraveled, very little is known about the associated 90degrees rotation process. One of the few rotation-specific mutations known is roulette (rlt) in which ommatidia rotate to a random degree, often more than 90degrees. Here we show that rlt is a rotation-specific allele of the inhibitory Egfr ligand Argos and that modulation of Egfr activity shows defects in ommatidial rotation. Our data indicate that, beside the Raf/MAPK cascade, the Ras effector Canoe/AF6 acts downstream of Egfr/Ras and provides a link from Egfr to cytoskeletal elements in this developmentally regulated cell motility process. We provide further evidence for an involvement of cadherins and nonmuscle myosin II as downstream components controlling rotation. In particular, the involvement of the cadherin Flamingo, a PCP gene, downstream of Egfr signaling provides the first link between PCP establishment and the Egfr pathway.