The distribution of calbindin, calretinin and parvalbumin immunoreactivity in the human thalamus
The distribution of calbindin, calretinin and parvalbumin immunoreactivity in the human thalamus
复制标题
DOI：
10.1016/s0891-0618(00)00060-0
复制
发表时间：
2000-07-01
影响因子：
2.8
通讯作者：
Faull, RLM
中科院分区：
文献类型：
--
作者：
Münkle, MC;Waldvogel, HJ;Faull, RLM
钙结合蛋白在哺乳动物中枢神经系统中呈异质性分布，是识别神经元群体的有用标志物。三种主要的钙结合蛋白-钙结合蛋白-D28 K（钙结合蛋白），钙视网膜蛋白和小清蛋白-的分布已被调查在8个神经正常的人丘脑使用标准的免疫组织化学技术。大多数丘脑核显示至少两个的三种钙结合蛋白的免疫反应性细胞体;唯一的核显示一种钙结合蛋白的免疫反应性是中央正中核（CM），这是小清蛋白阳性。总的来说，钙结合蛋白在人类丘脑中显示出互补的染色模式。一般而言，小清蛋白染色的最高密度在腹侧核组的组成核中（即在腹侧前、腹侧外侧和腹侧后核复合体中）以及在内侧和外侧膝状体核组中。中等密度的小清蛋白染色也存在于背内侧核（MD）区域。相比之下，钙结合蛋白和钙视黄蛋白免疫反应性均显示丘脑中致密染色的相似分布，这似乎补充了强烈的小清蛋白染色的模式。也就是说，钙结合蛋白和钙视网膜蛋白染色在嘴侧板内核组和MD的斑片状区域中最密集，这些区域显示非常低水平的小清蛋白染色。然而，钙结合蛋白和钙视网膜蛋白在腹侧核复合体以及内侧和外侧膝状体中也显示出低水平的染色，这与这些区域中强烈的小清蛋白染色重叠。这些结果表明，钙结合蛋白是不均匀分布在一个互补的方式在人类丘脑的核。它们为Jones最近提出的概念提供了进一步的支持（Jones，E.G.，1998.观点：丘脑组织的核心和基质。Neuroscience 85，331-345），灵长类丘脑由钙结合蛋白免疫反应细胞的基质和叠加的小清蛋白免疫反应细胞的核心组成，其可能具有不同的皮质投射模式。(C)2000 Elsevier Science B. V.保留所有权利。
Calcium-binding proteins show a heterogeneous distribution in the mammalian central nervous system and are useful markers for identifying neuronal populations. The distribution of the three major calcium-binding proteins - calbindin-D28k (calbindin), calretinin and parvalbumin - has been investigated in eight neurologically normal human thalami using standard immunohistochemical techniques. Most thalamic nuclei show immunoreactive cell bodies for at least two of the three calcium-binding proteins; the only nucleus showing immunoreactivity for one calcium-binding protein is the centre median nucleus (CM) which is parvalbumin-positive. Overall, the calcium-binding proteins show a complementary staining pattern in the human thalamus. In general terms, the highest density of parvalbumin staining is in the component nuclei of the ventral nuclear group (i.e. in the ventral anterior, ventral lateral and ventral posterior nuclear complexes) and in the medial and lateral geniculate nuclear groups. Moderate densities of parvalbumin staining are also present in regions of the mediodorsal nucleus (MD). By contrast, calbindin and calretinin immunoreactivity both show a similar distribution of dense staining in the thalamus which appears to complement the pattern of intense parvalbumin staining. That is, calbindin and calretinin staining is most dense in the rostral intralaminar nuclear group and in the patchy regions of the MD which show very low levels of parvalbumin staining. However, calbindin and calretinin also show low levels of staining in the ventral nuclear complex and in the medial and lateral geniculate bodies which overlaps with the intense parvalbumin staining in these reg;ions. These results show that the calcium-binding proteins are heterogeneously distributed in a complementary fashion within the nuclei of the human thalamus. They provide further support for the concept recently proposed by Jones (Jones, E.G., 1998. Viewpoint: the core and matrix of thalamic organization. Neuroscience 85, 331-345) that the primate thalamus comprises of a matrix of calbindin immunoreactive cells and a superimposed core of parvalbumin immunoreactive cells which may have differential patterns of cortical projections. (C) 2000 Elsevier Science B.V. All rights reserved.