Intrinsic regenerative potential of murine cochlear supporting cells.
Intrinsic regenerative potential of murine cochlear supporting cells.
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DOI：
10.1038/srep00026
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发表时间：
2011
影响因子：
4.6
通讯作者：
Heller S
中科院分区：
文献类型：
--
作者：
Sinkkonen ST;Chai R;Jan TA;Hartman BH;Laske RD;Gahlen F;Sinkkonen W;Cheng AG;Oshima K;Heller S
耳蜗再生电位的缺乏是导致听力损失永久性的主要原因。尽管在体内处于静止状态，但从新生儿耳蜗分离的非感觉细胞增殖并显示出在体外产生毛细胞样细胞的能力。然而，只有少数非感觉细胞衍生的集落产生毛细胞样细胞，这表明感觉祖细胞是增殖的非感觉细胞的亚群。在这里，我们纯化从新生小鼠耳蜗四个不同的非感觉细胞群体的荧光激活细胞分选（FACS）。所有四个群体显示增殖潜力，但只有较小的上皮嵴和支持细胞强烈地产生了毛细胞标记阳性细胞。这些结果表明，耳蜗支持细胞和细胞的小上皮嵴显示出强大的潜力去分化成prosensory细胞增殖和经历分化，以类似的方式天然的prosensory细胞的发展内耳。
The lack of cochlear regenerative potential is the main cause for the permanence of hearing loss. Albeit quiescent in vivo, dissociated non-sensory cells from the neonatal cochlea proliferate and show ability to generate hair cell-like cells in vitro. Only a few non-sensory cell-derived colonies, however, give rise to hair cell-like cells, suggesting that sensory progenitor cells are a subpopulation of proliferating non-sensory cells. Here we purify from the neonatal mouse cochlea four different non-sensory cell populations by fluorescence-activated cell sorting (FACS). All four populations displayed proliferative potential, but only lesser epithelial ridge and supporting cells robustly gave rise to hair cell marker-positive cells. These results suggest that cochlear supporting cells and cells of the lesser epithelial ridge show robust potential to de-differentiate into prosensory cells that proliferate and undergo differentiation in similar fashion to native prosensory cells of the developing inner ear.