Radiation Exposure Decreases the Quantity and Quality of Cardiac Stem Cells in Mice.
Radiation Exposure Decreases the Quantity and Quality of Cardiac Stem Cells in Mice.
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DOI：
10.1371/journal.pone.0152179
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Li TS
中科院分区：
文献类型：
--
作者：
Luo L;Urata Y;Yan C;Hasan AS;Goto S;Guo CY;Tou FF;Xie Y;Li TS
辐射暴露可能会增加心血管疾病的风险；然而，确切的分子/细胞机制仍不清楚。在本研究中，我们检验了辐射会损害心脏干细胞（CSC），从而增加未来心血管疾病风险的假设。成年C57BL/6小鼠接受3Gyγ射线照射，24小时后收集心脏组织用于进一步实验。尽管很少发现 c-kit 阳性细胞，但辐射暴露显着诱导心脏细胞凋亡和 DNA 损伤。从新鲜采集的心房组织中进行的 CSC 体外扩增显示，与健康小鼠相比，受辐射小鼠的 CSC 产量显着降低。通过 Ki-67 表达评估的 CSC 增殖活性在各组之间没有显着差异。然而，与健康对照相比，从受辐射小鼠扩增的CSC表现出显着较低的端粒酶活性、细胞核中更多的53BP1焦点、较低的c-kit表达和较高的CD90表达。此外，从受辐射小鼠中扩增出来的 CSC 在产生胰岛素样生长因子 1 方面的效力明显较差。我们的数据表明，辐射暴露显着降低了 CSC 的数量和质量，这可能作为预测未来心血管疾病风险的敏感生物参数。
Radiation exposure may increase cardiovascular disease risks; however, the precise molecular/cellular mechanisms remain unclear. In the present study, we examined the hypothesis that radiation impairs cardiac stem cells (CSCs), thereby contributing to future cardiovascular disease risks. Adult C57BL/6 mice were exposed to 3 Gy γ-rays, and heart tissues were collected 24 hours later for further experiments. Although c-kit-positive cells were rarely found, radiation exposure significantly induced apoptosis and DNA damage in the cells of the heart. The ex vivo expansion of CSCs from freshly harvested atrial tissues showed a significantly lower production of CSCs in irradiated mice compared with healthy mice. The proliferative activity of CSCs evaluated by Ki-67 expression was not significantly different between the groups. However, compared to the healthy control, CSCs expanded from irradiated mice showed significantly lower telomerase activity, more 53BP1 foci in the nuclei, lower expression of c-kit and higher expression of CD90. Furthermore, CSCs expanded from irradiated mice had significantly poorer potency in the production of insulin-like growth factor-1. Our data suggest that radiation exposure significantly decreases the quantity and quality of CSCs, which may serve as sensitive bio-parameters for predicting future cardiovascular disease risks.