miRNPs: a novel class of ribonucleoproteins containing numerous microRNAs
miRNPs: a novel class of ribonucleoproteins containing numerous microRNAs
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DOI：
10.1101/gad.974702
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发表时间：
2002-03-15
影响因子：
10.5
通讯作者：
Dreyfuss, G
中科院分区：
文献类型：
--
作者：
Mourelatos, Z;Dostie, J;Dreyfuss, G
Gemin 3是结合运动神经元存活（SMN）蛋白的DEAD盒RNA解旋酶，并且是SMN复合物的组分，SMN复合物还包括SMN、Gemin 2、Gemin 4、Gemin 5和Gemin 6。SMN蛋白减少导致脊髓性肌萎缩症（SMA），一种常见的神经退行性疾病。SMN复合物在多种核糖核蛋白（RNP）复合物的组装/重组中具有关键功能。在这里，我们报告说，Gemin 3和Gemin 4也在一个单独的复合物，包含elF 2C 2，Argonaute蛋白家族的成员。这种新的复合物是一个大的-15S RNP，包含许多microRNA（miRNA）。我们描述了40种miRNAs，其中一些与最近描述的人类miRNAs相同，这是一类小的内源性RNA。基因组序列预测，miRNA可能来源于具有形成茎环结构能力的较大前体。
Gemin3 is a DEAD-box RNA helicase that binds to the Survival of Motor Neurons (SMN) protein and is a component of the SMN complex, which also comprises SMN, Gemin2, Gemin4, Gemin5, and Gemin6. Reduction in SMN protein results in Spinal muscular atrophy (SMA), a common neurodegenerative disease. The SMN complex has critical functions in the assembly/restructuring of diverse ribonucleoprotein (RNP) complexes. Here we report that Gemin3 and Gemin4 are also in a separate complex that contains elF2C2, a member of the Argonaute protein family. This novel complex is a large -15S RNP that contains numerous microRNAs (miRNAs). We describe 40 miRNAs, a few of which are identical to recently described human miRNAs, a class of small endogenous RNAs. The genomic sequences predict that miRNAs are likely to be derived from larger precursors that have the capacity to form stem-loop structures.